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没有维度的板高度不适合比较色谱列
Ganna Dolinskaia1, Leonid M Blumberg2
1Retired, St. Petersburg, Russian Federation.
Journal of chromatography. A
|September 30, 2023
概括
无维板高度 (h) 是用于比较色谱列的不可靠指标. 新的客观指标,动力性能因子 (q) 和质量因子 (q_max),为不同的柱结构提供更好的性能评估.
科学领域:
- 分析化学 分析化学
- 染色学科学 染色学科学
背景情况:
- 无维板高度 (h) 对各种柱子内部结构 (开放式,包装式,单立式,柱子阵列) 定义不同.
- 板高度 (H) 和运动性能主要受到流通道尺寸的影响,而不是粒子或域大小等外部参数.
- 目前对h的指标是主观的,无法通过外部列分析来衡量,这限制了它们的实用性.
研究的目的:
- 为了证明无维板高度 (h) 在不同结构中评估柱子性能时的局限性.
- 引入和评估客观的性能指标:动力性能因子 (q) 和质量因子 (q_max).
- 提供一种可靠的方法来比较不同结构的染色体列的性能.
主要方法:
- 分析内部柱体结构尺寸如何影响流通道和整体运动性能.
- 评估之前引入的动力性能因子 (q) 和质量因子 (q_max).
- 对不同类型的柱子进行实际可行的性能比较,考虑超出q_max.
主要成果:
- 无维板高度 (h) 已被证明是一个不可测量的和主观的指标,不能准确地反映柱子的性能.
- 增加域大小 (d_dom) 独立于流通道尺寸 (d_chan) 可以人工减少h,而不会改善动力性能.
- 质量因子 (q_max) 被确定为一个合适的客观指标,用于比较不同结构的列.
结论:
- 无维板高度 (h) 不足以比较具有不同内部结构的柱子.
- 像质量因子 (q_max) 这样的客观指标为列比较提供了更可靠的基础.
- 实用的柱子性能取决于q_max以及其他因素,如压力耐受性和可实现的流通道尺寸.
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