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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Cell Specific Gene Expression01:58

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皮层:一个空间转录组学的全面存储库.

Guoliang Wang1,2, Song Wu1,3,2, Zhuang Xiong4

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概括

CROST是空间转录组学数据的新数据库,提供182个数据集和分析瘤相关基因的工具. 本资源增强了对组织生物学和疾病机制的理解,特别是在癌症中.

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科学领域:

  • 生命和健康科学 生命和健康科学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 由空间转录组测序驱动的空间奥米克,为复杂组织提供了新的见解.
  • 现有的空间转录组数据数据库有限,阻碍了全面的分析.
  • 需要一个集中存储库来整合和分析各种空间转录数据集.

研究的目的:

  • 建立一个完整且易于使用的空间转录基因数据数据库 - - CROST.
  • 为探索组织生物学和疾病提供标准化分析管道和集成的多omics数据.
  • 促进发现和理解与瘤相关的空间可变基因 (SVG) 及其在癌症中的作用.

主要方法:

  • 策划和整合了来自不同物种,器官和疾病的182个空间转录基因数据集.
  • 开发了一个标准化的数据处理管道和集成的单细胞RNA测序解卷.
  • 集成的多omics数据 (转录基因组,表观基因组,基因组) 用于对SVG进行全面分析.

主要成果:

  • 在CROST中包含182个空间转录基因数据集 (1033个子数据集) 和48043个与瘤相关的SVG.
  • 综合分析揭示了SVG的相关性,局部化,细胞间通信和生物功能.
  • 为数据注释和分析提供了两个在线工具 (基因组丰富分析和 SpatialAP).

结论:

  • CROST 作为空间转录学的宝贵资源,为组织结构和功能提供了前所未有的洞察力.
  • 数据库和综合分析为了解疾病机制,特别是瘤组织的理解提供了基础.
  • 克罗斯特为研究人员提供了浏览,分析,可视化和下载空间转录数据的工具,以便进一步发现.