Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Real Time RT-PCR02:57

Real Time RT-PCR

57.3K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Small and Prototypic: Dimethyl Ether in Lithiumorganics─Insights from Experimental Charge Density Studies.

Inorganic chemistry·2026
Same author

Polygenic, cell-envelope adaptations drive high-frequency daptomycin resistance in <i>Staphylococcus capitis</i> NRCS-A from neonatal sepsis and NEC.

Antimicrobial agents and chemotherapy·2026
Same author

Effects of a complex intervention on agitation and aggression in people living with dementia and mild cognitive impairment in shared-housing arrangements: results for a secondary outcome of the multicenter, cluster-randomized controlled DemWG study.

BMC psychiatry·2026
Same author

Heterochromatome wide analyses reveal MBD2 as a phase separation scaffold for heterochromatin compartmentalization and composition.

Nucleic acids research·2025
Same author

MeCP2-driven chromatin organization controls nuclear stiffness.

Communications biology·2025
Same author

Design, Synthesis, and Structural Evolution of Pseudo-Natural Product IDO1 Inhibitors and Degraders.

Angewandte Chemie (International ed. in English)·2025
Same journal

Editorial: Technologies for RNA Detection.

Bio-protocol·2026
Same journal

One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications.

Bio-protocol·2026
Same journal

Enhanced RNA-Seq Expression Profiling and Functional Enrichment in Non-model Organisms Using Custom Annotations.

Bio-protocol·2026
Same journal

Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types.

Bio-protocol·2026
Same journal

Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data.

Bio-protocol·2026
Same journal

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture.

Bio-protocol·2026
查看所有相关文章

相关实验视频

Updated: Jul 14, 2025

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
11:03

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

Published on: January 7, 2019

6.7K

在基于光的平台上量化蛋白质水平.

Hector Romero1, Annika Schmidt1, Cristina M Cardoso1

  • 1Department of Biology, Technical University of Darmstadt, Darmstadt, Germany.

Bio-protocol
|October 11, 2023
PubMed
概括
此摘要是机器生成的。

这项研究提出了一种新的方法,使用光强度量化单细胞中的蛋白质度. 这种技术可以在不同的细胞条件下准确地测量和比较蛋白质.

关键词:
传真 (FACS) 是一个事实.光是一种光效应.显微镜的使用方法量化 量化 量化 量化单细胞蛋白质含量 单细胞蛋白质水平西方涂抹是指西方涂抹.

更多相关视频

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

6.2K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K

相关实验视频

Last Updated: Jul 14, 2025

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
11:03

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

Published on: January 7, 2019

6.7K
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

6.2K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K

科学领域:

  • 细胞生物学 细胞生物学
  • 生物化学 生物化学
  • 生物物理学的生物物理.

背景情况:

  • 生物过程对蛋白质度敏感,具有固有的细胞可变性.
  • 精确的蛋白质定量对于理解细胞生理学和病理学至关重要.
  • 现有的方法,如西方污点 (平均人口) 或光强度 (相对) 有局限性.

研究的目的:

  • 开发一种方法来精确量化单细胞中的蛋白质度.
  • 建立一个校准曲线,将光强度与绝对蛋白质量相关联.
  • 为了使蛋白质水平在细胞和条件之间进行比较.

主要方法:

  • 净化和度的目标蛋白的确定.
  • 用感兴趣的蛋白质标记的细胞的光激活细胞分类 (FACS).
  • 使用纯化蛋白质进行校准,以将光强度与蛋白质量相关联.
  • 为普遍应用开发一个校准曲线.

主要成果:

  • 建立了一种结合蛋白质净化,FACS和强度测量的新方法.
  • 生成了一个校准曲线,使光强度与蛋白质度直接相关.
  • 该方法允许确定细胞下蛋白质度.

结论:

  • 这种技术提供了一种强大的方法来评估和比较单个细胞中的蛋白质度.
  • 一旦校准,它允许使用任何基于光的仪器量化蛋白质.
  • 该方法克服了以前用于单细胞蛋白质分析的技术的局限性.