竞争性相互作用促进了对抗微生物药物的耐药性发展
Luka Svet1, Ilse Parijs1, Simon Isphording2
1Department of Microbial and Molecular Systems, Centre of Microbial and Plant Genetics (CMPG), Leuven, Belgium.
Applied and environmental microbiology
|October 11, 2023
概括
细菌生物膜中的社会相互作用加速了抗菌素耐药性的演变. 与单一物种不同的是,混合物种社区使得Pseudomonas rhodesiae能够迅速发展出对硫法醇的耐药性,突出显示了社区对进化的影响.
科学领域:
- 微生物学 微生物学
- 进化生物学 进化生物学
- 细菌遗传学 细菌遗传学
背景情况:
- 抗菌素耐药性 (AMR) 的演变主要研究在孤立的细菌中,忽视了复杂的生物膜社区动态.
- 生物膜,密集的微生物群落,呈现独特的环境,社会相互作用可能会影响AMR.
- 了解混合物种生物膜中的AMR对于临床和工业环境至关重要.
研究的目的:
- 研究两种生物膜中的社会相互作用如何影响抗菌素耐药性的发展.
- 为了确定 *Raoultella terrigena* 对 *Pseudomonas rhodesiae* 适应硫法醇的影响.
- 阐明在社区环境中导致抗菌素耐药性的进化途径.
主要方法:
- 使用*Pseudomonas rhodesiae*和*Raoultella terrigena**构建和培育双种生物膜.
- 单种和双种生物膜暴露于抗菌素硫法醇的暴露.
- 耐药P. rhodesiae*分离物的全基因组测序.
- 实验排除了诸如增加种群规模,突变率和水平基因转移等因素.
主要成果:
- * Pseudomonas rhodesiae * 在双种生物膜中迅速发展出对硫酸的耐药性,但在单种生物膜中没有.
- 地的存在对于P. rhodesiae最初获得耐药性的存在至关重要.
- *P. rhodesiae*的抵抗机制并不直接依赖于*R. terrigena*,这表明间接的社区影响.
- 全基因组测序揭示了低频突变的复杂相互作用,而不是特定的耐药性热点.
结论:
- 社区的相互作用显著增强了细菌中抗菌素耐药性的演变.
- 生物膜中的社会动态为AMR创造了新的进化轨迹.
- 考虑微生物社区结构对于预测和管理抗菌素耐药性至关重要.
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