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Updated: Jul 13, 2025

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

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通过使用移动CRISPRi来研究病变发生过程中的Pseudomonas aeruginosa基因功能

Michelle A Yu1, Amy B Banta2,3, Ryan D Ward2,4

  • 1Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, Department of Medicine, University of California, San Francisco, CA, USA.

Methods in molecular biology (Clifton, N.J.)
|October 11, 2023
PubMed
概括

我们开发了Mobile-CRISPRi,这是一种用于像P. aeruginosa这样的细菌中的基因沉默的工具. 该系统允许在没有诱导剂的情况下进行部分基因敲除,有助于对基本基因和感染模型的研究.

关键词:
这就是CRISPR-Cas9的特征.克里斯普里是指克里斯普里.基本的基因 基本的基因鼠类肺炎模型中的小鼠肺炎伪omonas putida可以使用.伪蒙的注射器系统生物学 系统生物学

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Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants11:35

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