Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

23
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
23
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
CRISPR01:59

CRISPR

52.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Nuc domain electrostatics drive the trans cleavage activity of CRISPR-Cas12a.

Nucleic acids research·2026
Same author

Democratising high performance computing for bioinformatics through serverless cloud computing: A case study on CRISPR-Cas9 guide RNA design with Crackling Cloud.

PLoS computational biology·2025
Same author

Bioinformatic assessment of allergenicity, virulence, and secondary metabolites in Aspergillus species for industrial applications.

Computational biology and chemistry·2025
Same author

Privacy-hardened and hallucination-resistant synthetic data generation with logic-solvers.

Bioinformatics (Oxford, England)·2025
Same author

CRISPR-Cas12a REC2-Nuc interactions drive target-strand cleavage and constrain trans cleavage.

Nucleic acids research·2025
Same author

CapBuild: a cloud-native tool for adeno-associated virus capsid engineering.

Nucleic acids research·2025
Same journal

Thymidylate synthase inhibitory drugs induce p53-dependent pathways differently.

PloS one·2026
Same journal

Top-down and bottom-up attention for joint pattern classification and reconstruction.

PloS one·2026
Same journal

Short- and long-term scaling behavior of blood pressure and pulse arrival time during sleep in healthy controls and patients with obstructive sleep apnea.

PloS one·2026
Same journal

Double DQN-based secrecy energy efficiency and fairness performance in IRS-assisted NOMA systems with friendly jamming.

PloS one·2026
Same journal

10 recommendations for strengthening citizen science for improved societal and ecological outcomes: A co-produced analysis of challenges and opportunities in the 21st century.

PloS one·2026
Same journal

Paying in public: Peer effects, impression management, and willingness to pay on digital payment platforms.

PloS one·2026
查看所有相关文章

相关实验视频

Updated: Jul 13, 2025

CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

70.8K

预测CRISPR-Cas12a指南使用机器学习对目标的效率.

Aidan O'Brien1,2, Denis C Bauer2,3,4, Gaetan Burgio1

  • 1Division of Genome Science and Cancer and The Shine-Dalgarno Centre for RNA Innovation, The John Curtin School of Medical Research, College of Health and Medicine, The Australian National University, Canberra, ACT, Australia.

PloS one
|October 17, 2023
PubMed
概括
此摘要是机器生成的。

Cas12a基因组编辑显示,通过分析PAM位点附近的核酸偏差和不匹配,改善了指导RNA效率预测. 机器学习提高了超过15%的准确性,但需要更多的数据来进行可靠的目标外预测.

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.

更多相关视频

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
14:46

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Published on: May 28, 2015

11.1K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.8K

相关实验视频

Last Updated: Jul 13, 2025

CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

70.8K
Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
14:46

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Published on: May 28, 2015

11.1K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.8K
  • 生物信息学是一种生物信息学.

    • 背景情况:

    • 包括Cas9在内的CRISPR-Cas技术已经改变了生物研究.
    • Cas12a是Cas9的一个有希望的替代品,用于编辑富含AT的基因组.
    • 准确预测Cas12a的引导RNA效率仍然是一个挑战.

    结论:

    • 该研究提出了一种新的计算方法,以提高Cas12a指导RNA效率预测.
    • 这些发现强调了序列背景的重要性,特别是核酸偏差和PAM相互作用.
    • 进一步的研究需要更广泛的数据集和严格的基准测试,以便可靠地预测Cas12a效率和非目标效应.