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相关概念视频

Influence of Earth's Curvature and Atmospheric Refraction on Leveling01:26

Influence of Earth's Curvature and Atmospheric Refraction on Leveling

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During leveling, the Earth's curvature and atmospheric refraction introduce deviations in the line of sight from a true horizontal reference. When the line of sight is leveled, it remains perpendicular to the plumb line only at a single point. Beyond this, it deviates due to the Earth’s curvature, represented by the correction C. For a sight distance D, the deviation can be derived using the relationship:This relationship shows that the deviation increases quadratically with distance.
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Focusing of Light in the Eye01:16

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Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
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远程重新定位系统的对齐和特征.

Wenzhi Hong, Hugh Sparks, Chris Dunsby

    Applied optics
    |October 19, 2023
    PubMed
    概括

    这项研究调查了光学显微镜组件的错位如何影响远程重定焦性能. 适当的对齐对于最大限度地扩展轴向扫描范围,并保持偏光有限的成像质量至关重要.

    科学领域:

    • 光学显微镜的使用方法
    • 显微镜系统对齐方式
    • 远程重定焦技术 远程重定焦技术

    背景情况:

    • 远程重新聚焦使光学显微镜中的快速轴扫描能够在没有机械样本扰动的情况下实现.
    • 斜平面显微镜利用远程重新聚焦来成像样本内的倾斜平面.
    • 目标镜头放大对于实现广泛的远程重定焦范围至关重要.

    研究的目的:

    • 分析显微镜目标和管镜头轴向和侧向错位对远程重定焦性能的影响.
    • 量化二次管透镜焦距变化对系统偏差的影响.
    • 为了确定作为系统对齐的函数的衍射有限性能的体积.

    主要方法:

    • 执行了远程重定位系统的初始调整.
    • 引入了初级 (O1) 和二级 (O2) 目标以及管透镜 (TL1,TL2) 的控制式轴不对齐.
    • 测量点扩散函数 (PSF) 全宽度在半最大 (FWHM) 和集成光100纳米珠.
    • 在远程重定焦范围和图像位置上计算了轴向和侧向扭曲.

    主要成果:

    • 对PSF FWHM,集成光和扭曲的系统映射显示了对组件对齐的敏感性.
    • 错位明显改变了有效的远程重定焦范围和成像质量.

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  • 发现,衍射受限性能的体积强烈依赖于光学元件的精确对齐.
  • 结论:

    • 在光学显微镜中,精确对准目标和管状镜头对于优化远程重定焦性能至关重要.
    • 了解对齐效应可以预测和最大化可用的成像体积.
    • 这项工作为校准和改进远程重定焦系统提供了一个框架,用于诸如斜平面显微镜等应用.