相关实验视频

Updated: Jul 12, 2025

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

由转移素载荷的细胞外囊泡驱动的转基因效应

Vanesa Mattera¹, Federico Occhiuzzi¹, Jorge Correale^1,2

¹Departamento de Química Biológica, Instituto de Química y Fisicoquímica Biológica (IQUIFIB), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, CONICET, Buenos Aires, Argentina.

|October 20, 2023

概括

携带转基因的细胞外囊泡有效地将这种蛋白质输送到大脑,在脱髓化疾病模型中促进寡类细胞的成熟和复髓化.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

细胞外囊泡 (EVs) 在生理和病理状态下调解细胞间的细胞间通信.
对于髓来说至关重要的寡二细胞 (OLs) 在多发性硬化症 (MS) 等脱髓化疾病中受到损害.
转移素 (Tf) 对于铁的稳态和OL分化至关重要.

研究的目的:

研究EVs作为Tf传递到中枢神经系统 (CNS) 的鼻内 (IN) 纳米载体.
评估Tf装载EVs在促进复髓化中的治疗潜力.

主要方法:

在体外研究中使用了大鼠血EV和寡基细胞原生细胞 (OPC).
在体内研究中使用了cuprizone (CPZ) 诱导的脱髓化小鼠模型.
用Tf装载的EV被注射入鼻 (IN),并评估它们对大脑的透和影响.

主要成果:

装有Tf (EVTf) 的EV被OPC通过clathrin-caveolae和脂质通道内化,在体外促进OPC成熟.
在实体中,IN注射的充满Tf的EV在体内到达脑膜,增强了OPC分化,并在CPZ模型中加速了回髓化.
与可溶性Tf相比,EVs保护了Tf货物,并且需要较少的Tf进行复髓化.

结论:

电机作为有效的纳米载体,通过IN路线将Tf传送到CNS.
装有Tf的EVs促进OPC分化和复髓化,为脱髓化疾病提供了一个有前途的治疗策略.

关键词:

库普里兹脱离模式的模型外基因组是外基因组的组成部分.细胞外囊泡中的细胞外囊泡.鼻内注射鼻内注射鼻内注射骨髓蛋白是什么意思骨髓蛋白是什么意思复黄化是复黄化的一种.转移林是一种转移素.转移素受体 1 1 转移素受体

更多相关视频

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Published on: May 23, 2021

Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression

Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression

Published on: October 6, 2023

相关概念视频

The Early Endosome: Endocytosis of Transferrin

The Early Endosome: Endocytosis of Transferrin

Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...

Intralumenal Vesicles and Multivesicular Bodies

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...

Recycling Endosomes and Transcytosis

Recycling Endosomes and Transcytosis

The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...

Vesicular Tubular Clusters

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...

Receptor Downregulation in MVBs

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis