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相关概念视频

Viruses with RNA Genomes01:29

Viruses with RNA Genomes

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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
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相关实验视频

Updated: Jul 12, 2025

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
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从昆虫的内源性病毒元素中解脱昆虫的病毒组.

Paula Rozo-Lopez1, William Brewer2, Simon Käfer3

  • 1Department of Microbiology, University of Tennessee, Knoxville, TN, 37916, USA. plopez2@utk.edu.

BMC genomics
|October 24, 2023
PubMed
概括

在使用RNA测序时,昆虫基因组中的内源性病毒元素 (EVE) 可以被误认为是新病毒. 这项研究强调了EVE作为准确发现昆虫病毒多样性的重大挑战.

关键词:
虫 有助于虫的生长内源性病毒元素的内源性病毒元素.昆虫 昆虫是一种昆虫.在RNAseqqq中使用.病毒发现的发现.

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科学领域:

  • 病毒学 病毒学
  • 基因组学就是基因组学.
  • 昆虫学 昆虫学是一门学科.

背景情况:

  • 昆虫拥有大量尚未发现的病毒生物多样性.
  • 高通量RNA测序推进了昆虫病毒组研究.
  • 昆虫基因组中的内源性病毒元素 (EVE) 复杂化了使用RNA测序的病毒发现.

研究的目的:

  • 为了研究土豆虫的病毒组,Macrosiphum euphorbiae,一个重要的农业害虫和植物病原体载体.
  • 在虫的基因组中识别和区分外源病毒与内源病毒元素 (EVE).

主要方法:

  • 利用rRNA-depletedRNA测序来分析单个虫中的微生物群落.
  • 采用PCR查来评估当地虫群体中特定遗传病毒的流行程度.
  • 使用纳米孔测序与Illumina校正生成了M. euphorbiae的基因组组合草案,以检测转录活跃的EVE.

主要成果:

  • 检测到的读数来自昆虫特异性病毒,寄生虫病毒,植物致病病毒和通过RNAseq.的菌体.
  • 基因组组件显示,一些已识别的病毒序列实际上是虫基因组中存在的内源性病毒元素 (EVE).
  • 证明EVE在RNA测序数据中对虫病毒的错误识别有所贡献.

结论:

  • 内源性病毒元素 (EVE) 可以导致在昆虫RNA测序研究中错误识别病毒.
  • 对于准确地描述昆虫病毒的多样性来说,EVE的存在是一个广泛的挑战.
  • 建议在未来的昆虫病毒组研究中仔细考虑EVE.