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Updated: Jul 12, 2025

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
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Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

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斯托尔夫-记者:发现基因之间的基因.

Nicholas J Dimonaco1,2,3,4,5, Amanda Clare2, Kim Kenobi6

  • 1Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth SY23 3PD, Wales, UK.

Nucleic acids research
|October 28, 2023
PubMed
概括

StORF-Reporter在未注释的基因组区域中识别了缺失的 prokaryotic 基因. 这个工具发现了Stop-ORFs (StORFs),恢复了通常被标准注释方法遗漏的基因,提高了基因组注释的准确性.

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Using Coculture to Detect Chemically Mediated Interspecies Interactions

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A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

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相关概念视频

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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