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相关概念视频

Subcellular Fractionation01:32

Subcellular Fractionation

7.0K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
7.0K
Types of Receptors: Cell Surface Receptors01:28

Types of Receptors: Cell Surface Receptors

17.1K
Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
17.1K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.6K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.6K
Golgi Apparatus01:49

Golgi Apparatus

91.1K
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
91.1K
Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

104.6K
Overview
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Fluid Mosaic Model01:19

Fluid Mosaic Model

11.9K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
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相关实验视频

Updated: Jul 11, 2025

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
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In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

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组件亚细胞定位:细胞表面暴露

Anna Konovalova1

  • 1Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA. anna.konovalova@uth.tmc.edu.

Methods in molecular biology (Clifton, N.J.)
|November 6, 2023
PubMed
概括
此摘要是机器生成的。

确定细菌表面蛋白的位置至关重要. 这项研究提出了实验方法来检测表面暴露的脂蛋白在阴性细菌,因为计算预测是不可用的.

关键词:
生物化是生物化的一种.通过PEGylation进行化.蛋白质拓学 蛋白质拓学表面蛋白质溶解的作用整个细胞的点点点点.

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Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

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科学领域:

  • 微生物学 微生物学
  • 细菌细胞生物学 细菌细胞生物学
  • 蛋白质生物化学 蛋白质生物化学

背景情况:

  • 格拉姆阴性细菌具有外膜蛋白,包括不可分割的β-桶蛋白和脂蛋白.
  • 目前缺乏对表面暴露脂蛋白的准确预测,需要对其拓学的实验验证.
  • 了解蛋白质定位对于理解细菌外膜结构和功能至关重要.

研究的目的:

  • 描述和评估实验方法检测表面暴露的蛋白质在格兰氏阴性细菌.
  • 为研究细菌外膜蛋白位址的研究人员提供实用指南.
  • 为了解决缺少用于预测表面暴露脂蛋白的计算工具的问题.

主要方法:

  • 细胞表面蛋白质标记技术,以识别细菌外部可访问的蛋白质.
  • 评估蛋白质对细胞外蛋白酶或抗体的可访问性,作为表面暴露的指标.
  • 使用SpyTag/SpyCatcher系统,针对地检测和可视化表面定位的蛋白质.

主要成果:

  • 描述的方法提供了对表面暴露蛋白质的可靠检测的补充方法.
  • 实验验证证了标记,蛋白质酶/抗体可访问性和SpyTag/SpyCatcher用于脂蛋白拓学的有用性.
  • 这些技术提供了可靠的方法来确定脂蛋白是否暴露在细菌细胞表面.

结论:

  • 由于预测计算工具的局限性,实验方法对于确定细菌脂蛋白的表面暴露至关重要.
  • 细胞表面标记,蛋白酶/抗体可访问性测定和SpyTag/SpyCatcher系统的结合使蛋白质拓学的全面分析成为可能.
  • 这些经过验证的方法将有助于研究人员准确地描述格兰氏阴性细菌表面蛋白的局部.