开发一种基于改性银纳米颗粒的trientine量化分析方法
Mahsa Khodadadi1,2, Ali Shayanfar3,4
1Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
BMC chemistry
|November 8, 2023
概括
一种新的方法使用银纳米粒子 (AgNPs) 来量化Trientine (TETA). 这种具有成本效益的技术准确地测量了血中的TETA水平,为Wilson提供了更简单的替代方案.
科学领域:
- 分析化学 分析化学
- 纳米技术纳米技术
- 生物化学 生物化学
背景情况:
- 三氨酸 (N,N-bis(2-氨基乙烯) -1,2-乙二胺,TETA) 是一种对威尔逊病治疗至关重要的铜化剂.
- 由于TETA缺乏吸收紫外线的组,因此对直接量化提出了挑战.
- 银纳米粒子 (AgNPs) 为开发敏感分析方法提供独特的光学特性.
研究的目的:
- 开发和优化一种新的,具有成本效益的分析方法来量化TETA.
- 为了利用改性银纳米粒子 (AgNPs) 进行TETA检测.
- 为了验证该方法在等离子体等生物矩阵中的性能.
主要方法:
- 用硫酸修改AgNPs的方法.
- 使用响应表面方法来优化反应条件 (pH,时间,盐,AgNPs体积).
- 用光谱光度测量397 nm的AgNP吸收率的TETA诱导的变化.
主要成果:
- 为了定量TETA (10-40 ng/mL,R2=0.996),建立了一个线性校准曲线.
- 该方法表现出高灵敏度,检测极限 (LOD) 为 3 ng/mL,量化极限 (LOQ) 为 10 ng/mL.
- 该方法在血中显示出选择性,由于高灵敏度,常见离子体的干扰通过稀释减轻.
结论:
- 开发的基于AgNP的方法为TETA量化提供了一种敏感,选择性和低成本的方法.
- 这种技术适用于在血中测量TETA,而不需要昂贵的设备.
- 该方法为管理威尔逊病提供了一个实用的分析解决方案.
相关概念视频
Precipitation Titration Curve: Analysis
1.1K
The precipitation titration curve demonstrates the change in concentration of one reactant with the volume of titrant added. During the titration of chloride ions with silver nitrate, the precipitation titration curve is divided into three regions: before, at, and after the equivalence point. Before the equivalence point, low redissolution of the sparingly soluble silver chloride precipitate gives a low silver ion concentration. However, in the second region, representing the equivalence point,...
1.1K
Precipitation Titration: Endpoint Detection Methods
1.8K
In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...
In the Volhard method, a standard excess of AgNO3 is first added to the...
1.8K
Precipitation Titration: Overview
6.7K
Precipitation titration involves the reaction of a titrant and an analyte to generate an insoluble precipitate. While precipitation titration uses various precipitating agents, silver nitrate is the most common precipitating reagent; titrations involving Ag+ are called argentometric titrations. Usually, the endpoint in a precipitation titration can be detected by visual indicators.
A precipitation titration curve demonstrates the change in concentration of the titrant or analyte upon adding the...
A precipitation titration curve demonstrates the change in concentration of the titrant or analyte upon adding the...
6.7K
Titrimetric Methods: Types and Commonly Used Strategies
938
In chemistry, titrimetric methods are broadly classified into three types: volumetric, gravimetric, and coulometric. Volumetric titrations involve measuring the volume of a titrant of known concentration that is required to react completely with an analyte. In gravimetric titrations, the standard solution reacts with the analyte to form an insoluble precipitate, which is filtered, dried, and weighed. In coulometric titrations, current is applied to an electrochemical reaction until the reaction...
938
Precipitation Gravimetry
6.6K
Precipitation gravimetry is based on converting an analyte into a sparingly soluble precipitate, which is separated by filtration and weighed. An ideal precipitate should be pure, insoluble, of known composition, and easily filtered from the reaction mixture.
In determining nickel by gravimetric analysis, a precipitant of ethanolic dimethylglyoxime is added to a hot nickel salt solution. This is quickly followed by the dropwise addition of dilute ammonia solution until precipitation occurs. A...
In determining nickel by gravimetric analysis, a precipitant of ethanolic dimethylglyoxime is added to a hot nickel salt solution. This is quickly followed by the dropwise addition of dilute ammonia solution until precipitation occurs. A...
6.6K
EDTA: Indirect and Alkalimetric Titration
941
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
941


