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Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Updated: Jul 9, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

结构化照明对比转移功能用于高分辨率定量相位成像.

Sibi Chakravarthy Shanmugavel, Yunhui Zhu

|December 2, 2023

概括

此摘要是机器生成的。

我们开发了一种使用结构化照明显微镜 (SIM) 进行非光样本超高分辨率成像的新方法. 这种定量相位成像技术实现了亚衍射分辨率,提高了成像能力.

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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Phase Contrast and Differential Interference Contrast DIC Microscopy

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

相关实验视频

Last Updated: Jul 9, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Phase Contrast and Differential Interference Contrast DIC Microscopy

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

科学领域:

光学显微镜是一种光学显微镜.
超高分辨率成像成像技术
定量的相位成像成像技术

背景情况:

定量相成像 (QPI) 对于非光样本的无标签显微镜非常重要.
结构化照明显微镜 (SIM) 是一种超分辨率技术,主要用于光样本.
在非光标本的QPI中克服衍射极限仍然是一个挑战.

研究的目的:

应用结构化照明显微镜 (SIM) 进行非光样本的次衍射分辨率定量相位成像 (QPI).
为基于SIM的QPI开发一种新的对比传递函数 (CTF) 形式主义.
展示用于增强相位成像的确定性和非干扰度方法.

主要方法:

利用结构化照明显微镜 (SIM) 的新型应用来进行定量相位成像.
采用对比转移函数 (CTF) 形式主义,从失焦强度图像中提取高空间频率相位信息.
开发了一种无阶段解封算法,用于定量阶段重建.

主要成果:

在非光样本的定量相位成像中实现了亚衍射分辨率.
与传统成像相比,显示分辨率提高了两倍.
使用0.42 NA目标和660nm照明,人类脸细胞的侧面分辨率达到0.814μm.

结论:

提出的基于SIM的QPI技术成功地超过了非光样品的衍射极限.
该方法提供了一个实际的,非干涉测量设置与确定性算法.
这种方法显著提高了高分辨率定量相位成像能力.