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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Focusing of Light in the Eye01:16

Focusing of Light in the Eye

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Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Jul 8, 2025

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

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用于微内镜的近焦镜头的特征

Dominique Galvez1, Zhihan Hong1, Andrew D Rocha1

  • 1University of Arizona, Wyant College of Optical Sciences, Tucson, United States of America.

Journal of optical microsystems
|December 12, 2023
PubMed
概括
此摘要是机器生成的。

3D打印的微光学可以为微内镜提供高性能,近焦成像. 定制3D打印的三倍和双倍为水性环境中的小光度导航和疾病检测提供了卓越的光学解决方案.

关键词:
通过3D打印打印3D打印.内镜检查是指内镜检查.镜头设计 镜头设计微内镜 (microendoscope) 是一个微内镜.多模式成像技术多模式成像技术

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Video-rate Scanning Confocal Microscopy and Microendoscopy
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Video-rate Scanning Confocal Microscopy and Microendoscopy

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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

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相关实验视频

Last Updated: Jul 8, 2025

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

34.5K
Video-rate Scanning Confocal Microscopy and Microendoscopy
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Video-rate Scanning Confocal Microscopy and Microendoscopy

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科学领域:

  • 光学工程的光学工程.
  • 医学成像技术 医学成像技术
  • 增材制造 增材制造是一种增材制造.

背景情况:

  • 微内镜需要远距离光学用于导航和检测疾病在小体光线.
  • 现有的微光系统 (低于1mm) 在性能和成本方面存在局限性,而GRIN单片机则提供有限的调节性.
  • 3D打印的单立体光学系统为高性能,近焦成像提供了一个有希望的替代方案.

研究的目的:

  • 为了比较微内镜定制远端光学系统的光学性能.
  • 为了评估梯度指数 (GRIN) 单体与3D打印的单体双体和三体.
  • 评估3D打印是否适合在盐水中创建短工作距离 (WD) 微光学.

主要方法:

  • 设计和制造了三个定制的远距离光学系统:一个GRIN单片,一个3D打印的双片和一个3D打印的三片.
  • 在0.9%盐水中测试光学性能,名义工作距离分别为1.5mm,0.5mm和0.4mm.
  • 评估每个系统的视野深度 (DOF),以确定成像能力.

主要成果:

  • 格林单片机的性能受到光纤束继电器的限制,其射场深度 (DOF) 为0.9mm至1.6mm.
  • 3D打印的双层实现了0.71mm的相似的DOF.
  • 尽管工作距离很短,但3D打印的三联显示出最有限的DOF0.55mm,尽管工作距离很短.

结论:

  • 3D打印允许灵活设计具有aspheric表面的单质多元微光系统.
  • 3D打印光学可实现非常短的工作距离,这对于微内镜在崩的光线中至关重要.
  • 增材制造为开发先进的微内镜成像系统提供了一种可行和可适应的方法.