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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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易于扩展的多颜色基于DMD的结构化照明显微镜.

Daozheng Gong, Chufan Cai, Eli Strahilevitz

    Optics letters
    |December 22, 2023
    PubMed
    概括

    数字微镜设备使得结构化照明显微镜 (SIM) 的速度更快. 一种新的衍射格设置克服了波长分散,使复杂的生物研究能够进行多色超分辨率成像.

    科学领域:

    • 光学显微镜是一种光学显微镜.
    • 超高分辨率成像成像技术
    • 软物质物理学 软物质物理学

    背景情况:

    • 结构化照明显微镜 (SIM) 通过使用模式光提供超高分辨率成像.
    • 数字微镜设备 (DMD) 为SIM提供高速,经济高效的模式生成.
    • DMD 具有波长依赖的角分散,限制了多色SIM应用.

    研究的目的:

    • 使用DMD开发一个多色SIM设置.
    • 为了克服DMD在多色成像中的分散限制.
    • 为了展示生物样本的多色超高分辨率成像.

    主要方法:

    • 开发了一种多颜色的SIM系统,其中包含了一个DMD.
    • 采用了衍射来弥补DMD引起的波长分散.
    • 使用四种颜色通道对光珠和活细胞进行成像.

    主要成果:

    • 通过开发的DMD-SIM设置成功展示了多色超分辨率成像.
    • 在四个不同的颜色频道中实现了高质量的SIM图像.
    • 在标准校准样本和活生物标本上验证了系统的性能.

    结论:

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    • 一个衍射格有效地弥补了多色SIM中的DMD分散.
    • 开发的设置可以实现强大的多色超高分辨率成像.
    • 这种方法显著扩大了SIM用于研究复杂的多色生物系统的实用性.