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相关概念视频

Histone Modification02:32

Histone Modification

13.3K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.3K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

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The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.3K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K
Heterochromatin02:38

Heterochromatin

13.1K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
13.1K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K
Nucleosome Remodeling02:54

Nucleosome Remodeling

9.1K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
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相关实验视频

Updated: Jul 7, 2025

Analysis of Histone Antibody Specificity with Peptide Microarrays
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Analysis of Histone Antibody Specificity with Peptide Microarrays

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通过Polycomb复合体预测基因组甲基化,以极小的精度.

Moa J Lundkvist1, Ludvig Lizana2, Yuri B Schwartz1

  • 1Department of Molecular Biology, Umeå University, Umeå, Sweden.

Science advances
|December 22, 2023
PubMed
概括
此摘要是机器生成的。

聚合物抑制复合物2 (PRC2) 动态甲基化组素H3K27以抑制基因. 我们的模型显示H3K27甲基化取决于PRC2度和复制,并且表观遗传记忆不太可能在Drosophila中跨代传播.

更多相关视频

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

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相关实验视频

Last Updated: Jul 7, 2025

Analysis of Histone Antibody Specificity with Peptide Microarrays
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Analysis of Histone Antibody Specificity with Peptide Microarrays

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Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
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Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

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科学领域:

  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 发展生物学 发展生物学
  • 计算生物学 计算生物学

背景情况:

  • 聚合物系统通过聚合物抑制复合物2 (PRC2) 的介导,通过素H3 lysine 27三甲基化 (H3K27me3) 以表观遗传抑制发育基因.
  • 在细胞分裂过程中,H3K27me3的动态调节和遗传仍然不太清楚.

研究的目的:

  • 开发一个计算模型,预测Drosophila中的H3K27甲基化动态.
  • 研究影响H3K27甲基化和表观遗传记忆传输的因素.

主要方法:

  • 开发一个模拟H3K27甲基化并具有高时间和空间分辨率的计算模型.
  • 通过现有的H3K27me3.3.对PRC2度,复制频率和全性刺激进行分析.

主要成果:

  • 在分裂细胞中的H3K27甲基化水平是由PRC2度和复制频率决定的.
  • 体刺激增强了PRC2对发育基因的特异性.
  • 代际遗传的H3K27me3不太可能在Drosophila中通过快速的胚胎复制周期持续存在.

结论:

  • 计算模型提供了对H3K27甲基化和表观遗传基因调节的动态的见解.
  • 由于胚胎的快速复制,Drosophila的H3K27me3表观遗传记忆不会在几代人之间传播.
  • 该模型可适应用于研究小鼠和人类等其他生物体的表观遗传机制.