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相关概念视频

S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

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The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
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The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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Chromosome Replication02:31

Chromosome Replication

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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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Replication in Eukaryotes02:31

Replication in Eukaryotes

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Overview
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Negative Regulator Molecules01:23

Negative Regulator Molecules

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Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
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Updated: Jul 6, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

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主因KAP1协调转录和复制之间的时间控制.

Sarah Preston-Alp1, Italo Tempera1

  • 1The Wistar Institute, Philadelphia, PA, USA.

Trends in microbiology
|December 30, 2023
PubMed
概括
此摘要是机器生成的。

爱斯坦-巴尔病毒的重新激活需要精确的转录和复制时间. 研究人员发现,KAP1/EA-D/ATM通路是关键的,突出显示了宿主-病毒相互作用对于这一过程至关重要.

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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
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科学领域:

  • 病毒学 病毒学
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 爱斯坦-巴尔病毒 (EBV) 的重新激活是一个复杂的过程.
  • EBV的重新激活涉及病毒转录和DNA复制的协调时间控制.
  • 了解控制EBV重新激活的调控机制对于开发治疗策略至关重要.

研究的目的:

  • 确定调节EBV转录和复制的时间控制的关键宿主和病毒因素.
  • 阐明EBV重新激活背后的分子机制.

主要方法:

  • 研究了KAP1/EA-D/ATM轴在EBV重新激活中的作用.
  • 利用分子生物学技术分析宿主和病毒蛋白之间的相互作用.

主要成果:

  • 确定了KAP1/EA-D/ATM轴作为EBV转录和复制的关键调节器.
  • 证明了这一轴对于有效的EBV重新激活至关重要.
  • 突出了宿主和病毒因素在重新激活过程中的协作作用.

结论:

  • KAP1/EA-D/ATM轴是爱斯坦-巴尔病毒重新激活的关键调节器.
  • 对于有效的病毒再激活,宿主病毒因子协作是不可或缺的.
  • 这一发现为EBV延迟和重新激活的分子基础提供了新的见解.