无标签的DNA杂交检测使用高度敏感的纤维微腔生物传感器
Yao Wu1, Guiyu Wang1, Xiujuan Yu1,2
1College of Physical Science and Technology, Heilongjiang University, Harbin 150080, China.
Sensors (Basel, Switzerland)
|January 11, 2024
概括
这项研究介绍了一种用于超敏感DNA检测的新型光纤生物传感器. 这种无标签的设备具有高灵敏度和特异性,为先进的诊断和生物应用铺平了道路.
科学领域:
- 光子学和生物传感技术
- 生物技术和医疗诊断 生物技术和医疗诊断
背景情况:
- 无标签的生物感知对于实时生物分析至关重要.
- 光纤干扰仪提供高灵敏度的折射率测量.
研究的目的:
- 开发和展示一种新的无标签光纤生物传感器,用于敏感DNA检测.
- 在敏感度,特异性和可重复性方面调查生物传感器的性能.
主要方法:
- 使用单模纤维的偏移拼接制造微腔纤维马赫-泽恩德干扰仪的制造.
- 使用APTES对补充DNA (cDNA) 捕获的探针DNA (pDNA) 的固定.
- 通过光信号的变化对DNA杂交的实时监测.
主要成果:
- 实现了 -17,905 nm/RIU 的高灵敏度,用于折射率测量.
- 证明了0.32 nm/fM的DNA检测灵敏度,检测极限低至48.9 aM.
- 证实了DNA检测的高度可重复和特定性能.
结论:
- 成功开发了一种易于制造,超敏感且无标签的DNA生物传感器.
- 该生物传感器显示出在医学诊断,基因识别和环境监测方面的应用潜力很大.
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