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相关概念视频

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

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Updated: Jul 5, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
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基于CRISPR干扰的功能性小RNA基因组学

Gianluca Prezza1, Alexander J Westermann2,3,4

  • 1Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany.

Methods in molecular biology (Clifton, N.J.)
|January 13, 2024
PubMed
概括
此摘要是机器生成的。

通过向细菌的基因,CRISPR干扰 (CRISPRi) 能够对细菌小RNA (sRNA) 进行功能查. 这种方法克服了以前技术的局限性,促进了对各种细菌,包括肠道微生物群中的sRNA作用的研究.

关键词:
细菌菌菌类的存在克里斯普里是什么?克里斯普里是什么?这就是Cas12a.功能性基因组学是一种功能性基因组学.微生物群中的微生物群没有编码的RNA.他们是sRNARNA.

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科学领域:

  • 微生物学 微生物学
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 小RNAs (sRNAs) 是 prokaryotes 中的关键调节者,但它们的功能往往没有特征.
  • 传统的方法,如转子子突变发生,偏向于小基因,不足代表sRNA突变.
  • 克里斯普尔干扰 (CRISPRi) 为目标基因敲除提供了一种长度独立的方法.

研究的目的:

  • 为基于CRISPRi的细菌sRNA功能查提供详细的协议.
  • 为了证明CRISPRi用于识别sRNA函数的应用,使用Bacteroides thetaiotaomicron作为模型.
  • 为了能够系统地评估各种细菌物种中sRNA相关的表型.

主要方法:

  • 开发一种CRISPR干扰 (CRISPRi) 系统,用于向细菌sRNA的淘汰.
  • 设计和生成一个指导RNA库,针对基因间sRNA曲目.
  • 应用CRISPRi库用于适应性效应选在Bacteroides中的thetaiotaomicron.

主要成果:

  • 成功实施CRISPRi以有效地击败细菌sRNAs.
  • 识别与特定的sRNA敲击相关的健身效应.
  • 证明CRISPRi适用于对sRNA功能的无偏查.

结论:

  • 克里斯普干扰是细菌sRNA功能基因组学的一个强大而通用的工具.
  • 这一协议有助于在代表性不足的细菌种群中对sRNA进行系统研究,例如肠道微生物群.
  • 基于CRISPRi的功能查预计将大大推进细菌sRNA的研究.