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相关概念视频

Leaky Scanning02:28

Leaky Scanning

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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

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Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
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Retrovirus Life Cycles01:10

Retrovirus Life Cycles

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Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
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相关实验视频

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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

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核-RNA相互作用调节轮状病毒复制的发生.

Jey Hernández-Guzmán1, Carlos F Arias1, Susana López1

  • 1Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.

Journal of virology
|January 19, 2024
PubMed
概括
此摘要是机器生成的。

核蛋白通过结合病毒RNA的G-四重复结构来负面调节轮状病毒的复制. 抑制这种相互作用可以促进传染性轮状病毒的产生,从而揭示了病毒循环中的新调节机制.

关键词:
这是一种RNA结合蛋白.核的核是一种核.轮状病毒病毒的病毒.病毒宿主细胞的相互作用

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科学领域:

  • 病毒学 病毒学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 罗塔病毒是全球儿童胃肠炎的主要原因,其dRNA基因组位于蛋白质囊中.
  • 参与轮状病毒复制的细胞蛋白,特别是RNA结合蛋白,尚不清楚.
  • 核是一种多功能RNA结合蛋白,参与各种细胞过程.

研究的目的:

  • 研究核素在轮状病毒感染和复制中的作用.
  • 阐明核素影响轮状病毒产生的机制.
  • 通过了解宿主-病原体相互作用来确定轮状病毒的潜在治疗点.

主要方法:

  • 用RNA干扰来降低MA104细胞中的核素表达.
  • MA104细胞被AGRO100感染,AGRO100是一种G4结构形成化合物,以抑制核-RNA相互作用.
  • 在罗塔病毒RNA中确定了G-四重复 (G4) 序列,并通过寡核酸转染评估了它们的作用.

主要成果:

  • 核的淘汰显著增加了感染性 rhesus 轮状病毒 (RRV) 后代的产生 (6.3倍),病毒 mRNA 和基因组副本.
  • 抑制核-RNA与AGRO100的相互作用,导致病毒产量增加1.5倍.
  • 在所有11个RRV dsRNA段中都发现了G4序列,它们的模仿增加了病毒的产生.

结论:

  • 核素通过通过G-四重复结构与病毒RNA的直接相互作用来负面调节轮状病毒的复制.
  • 这种相互作用抑制了传染性病毒颗粒的产量,表明核素作为宿主限制因素.
  • 这些发现引入了轮状病毒复制中的新型调节机制,涉及宿主RNA结合蛋白和病毒RNA G4结构.