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Updated: Aug 2, 2026

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一种从冷保存组织中分离收缩平滑肌细胞的方法

Matheus L C Schultz1,2, Pranjal Seth1,2, Linda Kachmar2

  • 1Department of Biomedical Engineering, McGill University, Montreal, Quebec, Canada.

American journal of physiology. Cell physiology
|February 5, 2024
PubMed
概括

这项研究提出了一种新方案,用于从冷保存的组织中分离收缩平滑肌细胞 (SMC),克服新鲜组织需求的局限性. 开发的方法产生了可行的SMC,与培养细胞相比,它们更好地代表新鲜组织.

关键词:
细胞收缩性 细胞收缩性细胞缩短导致细胞缩短.单细胞研究的研究.顺肌细胞是平滑肌肉的细胞.

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相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...