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相关概念视频

Regulation of Expression Occurs at Multiple Steps02:24

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Structure of a Gene01:30

Structure of a Gene

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A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
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Cis-regulatory Sequences02:02

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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What is Gene Expression?01:36

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Updated: Jul 3, 2025

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从合成单细胞表达模式解读监管架构.

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此摘要是机器生成的。

为大规模并行报告测试 (MPRA) 开发"实验理论"可以提高对基因调节的理解. 这项工作模拟了MPRA数据,以优化实验设计,以准确地进行转录组的热力学建模.

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科学领域:

  • 基因组学就是基因组学.
  • 系统生物学 系统生物学
  • 计算生物学 计算生物学

背景情况:

  • 对于大多数被测序的基因,基因调节机制在很大程度上是未知的.
  • 对基因调节的定量理解对于解释生理和进化适应至关重要.
  • 大规模并行记者测试 (MPRA) 是研究转录组序列-表型关系的高通量实验.

研究的目的:

  • 开发一个解释MPRA数据的理论框架.
  • 了解生物和实验参数对MPRA结果的影响.
  • 优化MPRA实验设计,以实现精确的基因调节建模.

主要方法:

  • 使用平衡和失平衡模型生成合成单细胞基因表达数据.
  • 模仿MPRA总结统计数据 (信息足迹,表达式转移矩阵).
  • 使用精细的热力学模型与能量矩阵推断监管架构.

主要成果:

  • 模拟显示了各种参数对MPRA数据的显著影响.
  • 证明了MPRA实验设计的基础对特定热力学模型的优化.
  • 能够对突变表达特征映射进行详细检查.

结论:

  • 开发的理论增强了MPRA的多功能性和可扩展性.
  • 这种方法有助于创建正确的转录组热力学模型.
  • 该方法有助于设计更好的MPRA并探索监管演变.