矿物化脂肪衍生的干细胞组织的转录基因分析,用于性门疾病建模
Alyssa Brodeur1,2, Vincent Roy1,2, Lydia Touzel-Deschênes1,2
1Department of Surgery, Faculty of Medicine, Université Laval, Quebec City, QC G1V 5C3, Canada.
International journal of molecular sciences
|February 24, 2024
概括
组织工程的脂肪衍生的树皮/干细胞模仿形大动脉病 (CAVD) 在体外. 这些模型中的细胞外矩阵基因表达与CAVD有相似之处,突出了ECM.
科学领域:
- 生物医学工程 生物医学工程
- 心血管研究研究心血管研究
- 干细胞生物学 干细胞生物学
背景情况:
- 动脉疾病 (CAVD) 涉及动脉纤维化和矿化,导致狭窄和心力衰竭.
- 人类膜间歇细胞 (VIC) 很难获得用于CAVD的体外研究.
- 脂肪基衍生干细胞 (ASCs) 为患者提供了特定的,易于获得的疾病建模替代方案.
研究的目的:
- 进行组织工程,无支架ASC嵌入矿化板的转录组分析.
- 研究细胞外基质 (ECM) 在ASC驱动的矿化中的作用.
- 将基因表达特征与人类CAVD数据集进行比较.
主要方法:
- 组织工程ASC嵌入矿物化板块的散装RNA测序.
- 生物信息学和基因组丰富分析.
- 与公开可用的CAVD基因表达数据进行比较.
主要成果:
- 在工程组织中观察到的ECM组织基因的上调.
- 工程组织和CAVD患者之间心血管和ECM相关基因表达的相似性.
- ECM促进RUNX2激活,这是CAVD的一个关键因素.
结论:
- 组织工程ASC表为CAVD研究提供了可行的体外模型.
- 在CAVD病原发生过程中,ECM起着重要作用.
- 这种模型可能有助于开发新型的大动脉替代疗法.
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