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相关概念视频

Spermatogenesis01:41

Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Sperm Structure and Semen Composition01:22

Sperm Structure and Semen Composition

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During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
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Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
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基于特征的3D+t描述器对过度激活的人类精子的节拍模式.

Haydee O Hernández1,2, Fernando Montoya2, Paul Hernández-Herrera2,3

  • 1Posgrado en Ciencia e Ingeniería de la Computación, Universidad Nacional Autónoma de México, UNAM, Ciudad de México, Mexico.

Heliyon
|March 6, 2024
PubMed
概括

研究人员开发了一种新的3D方法来分析精子运动性,准确地区分对受精至关重要的过度活化的精子. 这一进步有助于生育研究,为精子鞭毛跳动模式提供了可靠的分类.

关键词:
三维显微镜的3D显微镜3D+t人类精子运动率过度活化的精子多平面成像多平面成像时间空间的模式.精子的鞭子是精子的鞭子.没有监督的分类.

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科学领域:

  • 生殖生物学 生殖生物学
  • 生物物理学的生物物理.
  • 计算生物学 计算生物学

背景情况:

  • 精子鞭状运动对于受精至关重要.
  • 容量诱导精子运动的变化,导致卵子受精所需的过度激活.
  • 过度活化运动的特点是特定的不对称的鞭毛曲,但它的3D模式仍然没有特征.

研究的目的:

  • 为了描述过度活化的精子的3D运动模式.
  • 开发一种用于分类3D精子鞭毛鞭子击打模式的方法.
  • 为精子分析中的未来深度学习应用提供基础真相参考.

主要方法:

  • 使用多焦图像采集系统进行3D+t精子鞭毛追踪.
  • 开发了一个基于特征的向量,用圆的信封描述时空运动模式.
  • 应用了开发的描述符来分类人类精子运动模式.

主要成果:

  • 成功地区分了非过激活和过激活的3D精子运动模式.
  • 基于3D特征的描述器为深度神经网络训练提供了潜在的基础真相.
  • 证明了3D描述器在分类人类精子细胞中的有效性.

结论:

  • 这项研究首次描述了3D过度激活的精子运动模式.
  • 开发的3D描述器为自动化精子分类提供了一个框架.
  • 这项研究促进了对人类和动物生育能力的理解.