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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jul 1, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

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使用编码搜索焦点扫描进行快速地形光学成像.

Narcís Vilar1,2, Roger Artigas1, Martí Duocastella3

  • 1Sensofar Tech S.L, Parc audiovisual de Catalunya, BV-1274 km 1, 08225, Terrassa, Spain.

Nature communications
|March 7, 2024
PubMed
概括

这项研究引入了一种新的地形成像方法,通过使用调制照明和更少的图像来显著加快测量. 这一突破使动态样本的实时,高速3D表面映射成为可能.

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科学领域:

  • 光学和光子学 在光学和光子学.
  • 计量学 计量学 计量学
  • 表面科学是一门学科.

背景情况:

  • 地形成像对于精密工程和质量控制中的微/纳米尺度测量至关重要.
  • 目前的方法需要耗时的焦点堆,限制测量到静态样本.
  • 从样本中快速提取定量信息仍然是光学领域的一个核心挑战.

研究的目的:

  • 开发一种更快的地形成像方法.
  • 为了实现动态和大规模样本的实时表征.
  • 克服传统的焦点堆式成像技术的局限性.

主要方法:

  • 这是一种新的方法,使用了一组减少的图像,每个图像都在完整的焦点扫描中集成.
  • 在图像曝光期间同步调节照明.
  • 通过设计的调制序列,对象高度图的明确重建.

主要成果:

  • 实现了拓成像速度的数量级增加.
  • 在100微米范围内展示了微米以下的地形成像.
  • 在静态和动态系统中达到高达每秒67个地形的成像速度.

结论:

  • 拟议的技术为光学计量学提供了一个范式的转变.
  • 移动样本的高速实时表征现在是可行的.
  • 易于实施和速度使这种方法广泛适用.