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Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Updated: Sep 13, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

快照量化相位成像与声光色异常控制

Christos Alexandropoulos¹, Laura Rodríguez-Suñé¹, Martí Duocastella^1,2

¹Department of Applied Physics, Universitat de Barcelona, C/Martí i Franquès 1, 08028 Barcelona, Spain.

Sensors (Basel, Switzerland)

|July 30, 2025

概括

这项研究引入了一种新系统,用于快速定量相位成像,使用多色照明和声光失焦. 它使动态样本的实时相位成像能够以高速速度进行.

关键词:

声光学光学学声光学照明编码的编码.没有标签的显微镜.液晶镜头是一种液态镜头.运输强度方程的运输.

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Published on: January 28, 2019

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

相关实验视频

Last Updated: Sep 13, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

科学领域:

光学和成像科学科学光学和成像科学
生物物理学的生物物理.
显微镜的使用方法

背景情况:

定量相位成像 (QPI) 使用强度运输方程 (TIE) 来分析低对比度样本.
当前的QPI方法在快速失焦平面选择和顺序图像采集方面面临挑战,这限制了动态事件的实时成像.

研究的目的:

开发一个快速,用户调整的失焦和快照阶段检索系统.
克服传统QPI中的顺序图像采集的局限性.

主要方法:

结合多色脉冲照明与声光失焦,用于微秒级的色态偏差控制.
在单个摄像机曝光中获取相位图信息,使用每个平面的不同颜色.

主要成果:

展示了一个自由移动的微生物的活相成像.
实现了每秒150的相位重建速度,受到相机率的限制.

结论:

开发的系统可以实现高速,单次获取量化相位成像.
这一进步促进了动态生物过程的实时表征.