Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

<i>Cis</i>-regulatory effects of transposable element insertion/absence polymorphisms in the <i>Brassica napus</i> population.

Horticulture research·2026
Same author

A small-scale CRISPR mutant library in rapeseed of commercial cultivar Zhongshuang 11.

Horticulture research·2026
Same author

Glucosinolates in <i>Brassica</i> Species: Biosynthesis, Regulation, and Molecular Breeding.

International journal of molecular sciences·2026
Same author

Gelatin/Lignin Hydrogel Loaded with Mesenchymal Stem Cell-Derived Exosomes Enriched in Microrna-185 Inhibits Progression of Oral Cancer.

Pharmaceutics·2026
Same author

BnLEA4 and BnMYB96 target BnLTP2 to enhance drought tolerance and oil accumulation in Brassica napus L.

The Plant journal : for cell and molecular biology·2026
Same author

<i>Poria cocos</i> Attenuates LPS/D-Galactosamine-Induced Acute Liver Failure in Rats: An Integrative Exploratory Study Combining Network Pharmacology and In Vivo Validation.

International journal of molecular sciences·2026

相关实验视频

Updated: May 12, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

13.9K

植物功能性基因组学基于高通量CRISPR库淘汰查:一个前景

Jianjie He1,2, Can Zeng1,2, Maoteng Li1,2

  • 1Department of Biotechnology College of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China.

Advanced genetics (Hoboken, N.J.)
|March 11, 2024
PubMed
概括

这项研究探讨了用于植物功能基因组学的CRISPR-Cas9基因编辑. 它强调了CRISPR库作为高通量基因淘汰和在未表征的植物DNA序列中发现功能的强大工具.

关键词:
这里是CRISPR图书馆.功能性基因组学 功能性基因组学高吞吐量,具有高吞吐量.淘汰赛 淘汰赛 是一个淘汰赛.突变的收集 突变的收集

更多相关视频

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
00:09

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

21.9K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

14.6K

相关实验视频

Last Updated: May 12, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

13.9K
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
00:09

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

21.9K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

14.6K

科学领域:

  • 植物生物学 植物生物学
  • 基因组学就是基因组学.
  • 分子生物学分子生物学

背景情况:

  • 后基因组时代的植物生物学专注于基因功能注释.
  • 已建立的突变种群拥有先进的功能基因组学.
  • 许多植物基因组功能和调节网络仍然未知.

研究的目的:

  • 为了比较当前的植物功能基因组学方法.
  • 讨论CRISPR-Cas9用于多目标DNA扰乱和克服基因冗余的策略.
  • 总结CRISPR库在植物中的应用,用于基因淘汰和功能发现.

主要方法:

  • 植物功能基因组学技术的比较分析.
  • 针对性DNA修饰的CRISPR-Cas9系统.
  • 在CRISPR库中选用于高通量分析.

主要成果:

  • 在功能研究中,CRISPR-Cas9提供了多功能基因组操纵.
  • 多目标策略有效地解决了基因冗余问题.
  • 克里斯普尔图书馆可以实现高效的基因淘汰和功能发现.

结论:

  • 克里斯普技术显著增强了植物功能基因组学研究.
  • 克里斯普尔图书馆选是探索未表征植物DNA的关键策略.
  • 未来的工作重点应该是优化CRISPR查,以更广泛的基因组应用.