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Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

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一个基于水力动力学的双重功能微流体芯片,用于高吞吐量分辨瘤细胞.

Yu-Jia Wei¹, Xing Wei¹, Xuan Zhang¹

¹Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.

|March 20, 2024

概括

这项研究引入了一种微流体芯片,该芯片有效地使用水力动力学力量将瘤细胞 (TC) 与全血细胞分离. 该设备通过首先隔离TC,然后去除剩余的血细胞来实现高纯度.

关键词:

CEA微通道 CEA微通道净化微通道的微通道瘤细胞是一种瘤细胞.这是全血,全血.

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科学领域:

生物医学工程生物医学工程
微流体学微流体学
细胞分离技术细胞分离技术

背景情况:

从全血中准确分离瘤细胞对于癌症诊断和治疗监测至关重要.
现有的方法经常面临效率,纯度和吞吐量方面的挑战.
微流体装置为精确的细胞操纵和分离提供了一个有前途的平台.

研究的目的:

设计和验证一种基于水力动力学的新型微流体芯片,以有效地从全血中分离和净化瘤细胞.
为了利用微流体原理进行尺寸依赖的细胞分类,从而实现高纯度的瘤细胞隔离.

主要方法:

一个微流体芯片整合了收缩扩张阵列 (CEA) 微通道,用于基于尺寸依赖的提升力进行初始瘤细胞分离.
随后的水力动力过 (HDF) 装置用于去除剩余的血细胞,确保分离的瘤细胞的高纯度.
利用光粒子和MCF-7癌细胞来模拟和验证分离和净化性能.

主要成果:

对于较大的颗粒/细胞,CEA微通道显示出高的分离效率 (例如,19.3μm颗粒的98.7%,MCF-7细胞的96.1%).
对较小的血细胞实现了显著的去除率 (例如,4.5μm颗粒的96.2%,红细胞的96.2%,白细胞的98.7%).
组合芯片实现了约95.3%的瘤细胞分离率和超过99.99%的血液细胞去除.

结论:

开发的水力动力微流体芯片有效地将瘤细胞与高纯度的全血分离.
采用CEA和HDF的两阶段设计,为需要精确的细胞隔离的临床应用提供了强大的解决方案.
这项技术有可能推进液体活检技术和个性化癌症治疗.