Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Flow Cytometry01:23

Flow Cytometry

13.0K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Intercellular nanotube-mediated mitochondrial transfer enhances T cell metabolic fitness and antitumor efficacy.

Cell·2024
Same author

LIM-domain-only 4 (LMO4) enhances CD8<sup>+</sup> T-cell stemness and tumor rejection by boosting IL-21-STAT3 signaling.

Signal transduction and targeted therapy·2024
Same author

Multiparametric Analysis of Apoptosis by Flow Cytometry.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Laser Sources for Traditional and Spectral Flow Cytometry.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Flow cytometry and cell sorting.

Frontiers in medicine·2023
Same author

Mammalian Chromosome Analysis and Sorting by Flow Cytometry.

Current protocols·2023
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
查看所有相关文章

相关实验视频

Updated: Jun 29, 2025

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
13:05

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

52.3K

流动细胞计操作的基本原理: 制作您自己的流动细胞计

William G Telford1

  • 1Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. telfordw@mail.nih.gov.

Methods in molecular biology (Clifton, N.J.)
|March 25, 2024
PubMed
概括
此摘要是机器生成的。

本章介绍了流细胞计操作的基本原理. 它使用自制的流量细胞计来增强生物医学教育和对这一关键技术的理解.

关键词:
放大器的放大器模拟到数字转换器 模拟到数字转换器带通波器的波器带通波器的波器.二氧化镜镜子的二氧化镜子数字信号处理器数字信号处理器数字化器的数字化器流动细胞计量流动细胞计量水力动力聚焦是指水力动力聚焦.激光 激光器 激光器 激光器光电极 photodiode 的使用情况.摄影多重管中的多重管.预放大器的预放大器

更多相关视频

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
11:32

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Published on: September 28, 2016

13.3K
Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
06:22

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Published on: January 9, 2019

13.0K

相关实验视频

Last Updated: Jun 29, 2025

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
13:05

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

52.3K
Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
11:32

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Published on: September 28, 2016

13.3K
Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
06:22

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Published on: January 9, 2019

13.0K

科学领域:

  • 生物医学工程 生物医学工程
  • 免疫学 免疫学 免疫学
  • 教育技术的教育技术

背景情况:

  • 流细胞计是生物医学研究中的重要工具,特别是在免疫系统研究中.
  • 越来越复杂的仪器常常导致人们对基本操作原理的理解有差距.
  • 教育方面忽视了基本的流细胞计概念是一个广泛的问题.

研究的目的:

  • 描述流细胞计的基本操作原理.
  • 为了引入一个动手的教育模式,学习流动细胞计.
  • 为开发类似的教育系统提供框架.

主要方法:

  • 利用"制作你自己的流动细胞计"项目作为一个实用的学习工具.
  • 从一个套件组装一个功能性流动细胞计.
  • 通过实践项目展示关键的流细胞计原理.

主要成果:

  • 学生可以组装一个工作流细胞计.
  • 该项目有效地说明了核心流细胞计的操作原则.
  • 这种方法提高了对复杂的生物医学技术的理解.

结论:

  • 实践项目,如"制造你自己的流动细胞计"是有效的生物医学教育.
  • 构建流动细胞计提供了对其基本操作的实用见解.
  • 这个模型可以激发进一步开发可访问的流细胞计学学习工具.