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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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通过流细胞计对亡的多参数分析.

William G Telford1

  • 1Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. telfordw@mail.nih.gov.

Methods in molecular biology (Clifton, N.J.)
|March 25, 2024
PubMed
概括

流细胞计是研究细胞死亡的关键工具. 多参数分析结合了多个亡标记,以更深入地了解细胞死亡的进展,提供了比单个分析更多的信息.

科学领域:

  • 细胞生物学 细胞生物学
  • 免疫学 免疫学 免疫学
  • 生物化学 生物化学

背景情况:

  • 流细胞计是检测和量化哺乳动物细胞中细胞亡和细胞死亡的标准.
  • 它的多参数性质使得在单个样本中同时分析多个apoptotic特征.
  • 这有助于全面了解复杂的亡死亡过程.

研究的目的:

  • 提出创建多参数流细胞计试验的方法.
  • 为了将单个亡标志物 (酶激活,素V结合,膜透性) 的测定组合到单个,全面的分析中.
  • 为了加深对细胞死亡机制的洞察力.

主要方法:

  • 开发结合多个亡检测测定量的协议.
  • 使用流式细胞计量来同时标记和分析各种亡标记物.
  • 专注于酶激活,附录素V结合和细胞膜透性的测试.

主要成果:

  • 多参数测试比单参数测试提供了更多的信息.
  • 同时分析多个亡特征,可以更深入地了解细胞死亡.
  • 描述的多色方法与标准流量细胞计兼容.

结论:

关键词:
7 - 氨基动因组胺素D附录V 附录V 是一个附录.细胞灭亡 (apoptosis) 是一种死亡的过程.卡斯帕塞斯 (Caspase) 是一个协价活力探针 协价活力探针流动细胞计量流动细胞计量化物卡斯帕斯基底物霍克斯特染料公司的染料尸体灭 (Necroptosis) 是一种死亡的过程.太平洋蓝色 太平洋蓝色酸 (Propidium iodide) 是一种酸的化合物.热灭菌的发生.

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  • 多参数流细胞计是研究亡的一个强大且易于使用的方法.
  • 结合多种测试可以提高对细胞死亡复杂性的理解.
  • 这些方法为细胞死亡研究提供了更具信息性的方法.