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Updated: Jun 29, 2025

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

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使用氨基的降解甲基化量化peptidomics.

Alexandre K Tashima1, Leandro M de Castro2, Lloyd D Fricker3

  • 1Department of Biochemistry, Escola Paulista de Medicina, Federal University of Sao Paulo, Sao Paulo, SP, Brazil. aktashima@unifesp.br.

Methods in molecular biology (Clifton, N.J.)
|March 29, 2024
PubMed
概括

这项研究提出了一种新型的定量体学方法,使用甲和核化来标记. 这种技术允许基于质谱的量化测量五种同位素上不同的形式.

关键词:
博化物化物甲甲是一种形式的甲.同位素标签的标签

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Mass Spectrometry of Amines01:19

Mass Spectrometry of Amines

In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule: a molecule with an odd number of nitrogen atoms produces a parent ion with an odd molecular weight. The remaining fragments have an even mass.
Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit...
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