Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

2.0K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.0K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

3.5K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.5K
Drugs that Destabilize Microtubules01:10

Drugs that Destabilize Microtubules

2.0K
Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...
2.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Influence of Joint Structure in Multibody Model on Behavior Analysis of Crash Test Dummy.

Stapp car crash journal·2026
Same author

GAK antagonises ROCK-dependent regulation of actomyosin dynamics.

Journal of cell science·2026
Same author

Myeloid-specific Exoc5 deficiency develops renal inflammation and hypertension.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

[Rectosigmoid Colon Cancer with Unresectable Liver Metastases That Responded Remarkably to Bevacizumab-Combined Chemotherapy-A Case Report].

Gan to kagaku ryoho. Cancer & chemotherapy·2025
Same author

[A Case of Pathological Complete Response Post‒Pembrolizumab Therapy for Recurrent Liver Metastasis of Ascending Colon Cancer].

Gan to kagaku ryoho. Cancer & chemotherapy·2025
Same author

Expanding diverse horizons in smooth muscle research: a symposium review of "Muscle and Pathology/Health: Frontiers of Translational Research" at the 66th Annual Meeting of Japan Society of Smooth Muscle Research.

Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi·2025

相关实验视频

Updated: Jun 28, 2025

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles
06:58

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Published on: October 18, 2024

664

阿贝马西利布和瓦库林-1通过加速自流量来降低聚合物倾向的TDP-43积累.

Yoshinori Tanaka1, Lina Kozuma1, Hirotsugu Hino2

  • 1Biochemistry Unit, Faculty of Veterinary Medicine, Okayama University of Science, Imabari-shi, Ehime, Japan.

Biochemistry and biophysics reports
|April 10, 2024
PubMed
概括

亚贝马西克利布和真空林-1通过增强自细胞形成和与溶酶体的融合来加速聚合物倾向的TDP-43的细胞降解,这一过程取决于PI(3) P.

关键词:
阿贝马西克利布 (Abemaciclib) 是一个女性.自流是自流的自流.PI(3) P3) P3) P3) P3) P3) P3) P3) P3) P) P) P) P) P) P) P)在TDP-43中使用.瓦库林-1是一种真空.

更多相关视频

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

7.2K
Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain
10:08

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain

Published on: August 28, 2012

11.8K

相关实验视频

Last Updated: Jun 28, 2025

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles
06:58

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Published on: October 18, 2024

664
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

7.2K
Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain
10:08

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain

Published on: August 28, 2012

11.8K

科学领域:

  • 细胞生物学 细胞生物学
  • 神经科学是一个神经科学.
  • 分子生物学分子生物学

背景情况:

  • 自是一种关键的细胞过程,用于降解不需要的物质,包括易于聚合的TDP-43.
  • TDP-43聚合涉及神经退行性疾病,如肌缩侧面硬化症和前叶退化.
  • 已知abemaciclib (Abe) 和vacuolin-1 (Vac) 诱导了自细胞-溶酶体结构,这表明它们在自细胞中发挥了作用.

研究的目的:

  • 调查Abemaciclib和真空素-1是否加速自流并减少TDP-43的积累.
  • 阐明Abe和Vac影响自菌体-溶酶体融合和TDP-43降解的机制.

主要方法:

  • 使用的SH-SY5Y神经母细胞瘤细胞表达自流和溶酶体标记物 (GFP-LC3-RFP-LC3ΔG,mCherry-LC3,LAMP1-GFP).
  • 在Abe和Vac治疗后评估了自细胞形成,溶酶体局部化和TDP-43水平.
  • 采用PI(3) P记者测定和VPS34抑制剂 (沃特曼宁) 来检查途径的参与.

主要成果:

  • 根据剂量,Abe和Vac降低了GFP/RFP比率,表明自流加速.
  • 这些疗法增加了与溶解体结合的自细胞标记物,并增强了溶解体上的PI(3) P信号.
  • 阿贝和Vac治疗以自依赖的方式抑制了TDP-43的积累,而沃特曼宁则阻止了这些效应.

结论:

  • 阿贝马西利布和真空-1促进了易于聚合的TDP-43.3的降解.
  • 这些药物通过增加PI(3) P形成来增强自,从而加速自细胞形成和溶酶体融合.
  • 这些发现表明Abe和Vac在与TDP-43聚合相关的神经退行性疾病中的治疗潜力.