Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spatially resolved profiling of extracellular vesicles in tissues with Spatial-EV-seq.

Nature biotechnology·2026
Same author

Cyclic transformation of stable/metastable nucleic acid structures enables dynamic monitoring of ATP in living cells.

Chemical science·2026
Same author

Dual-Spatially Confined Assembly of DNA Nanowall Stiffens Tumor Cells to Enhance Adoptive T-Cell Immunotherapy.

Journal of the American Chemical Society·2026
Same author

Tracing Tumor-Derived Extracellular Vesicle Matrix Metalloproteinase 14 Using Dual-Target Orthogonal Barcoding-Based Microscale Thermophoretic Assays.

ACS nano·2026
Same author

RENAL-CHIP: Rejection Evaluation via Non-Invasive Analysis of Circulating Podocytes With Herringbone-Chip Isolation Platform.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Metabolic RNA Labeling-Enabled Time-Resolved Single-Cell RNA Sequencing.

Accounts of chemical research·2026

相关实验视频

Updated: Jun 28, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K

使用数字微流体学进行高度复合,吞吐量和高效的单细胞蛋白质分析.

Linfeng Cai1, Li Lin1, Shiyan Lin1

  • 1The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, the Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, Department of Chemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.

Small methods
|April 12, 2024
PubMed
概括
此摘要是机器生成的。

一种新的数字微流体蛋白质测序 (DMF-Protein-seq) 方法可以实现高通量单细胞蛋白质分析. 这种基于DNA的方法增强了细胞表型调查和药物监测能力.

关键词:
数字微流体技术的发展检测蛋白质检测蛋白质的检测测序的测序是指测序的测序.一个单细胞的单细胞.

更多相关视频

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

10.6K
Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.3K

相关实验视频

Last Updated: Jun 28, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K
A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

10.6K
Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.3K

科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 蛋白质对细胞功能至关重要,使敏感检测对于了解细胞表型至关重要.
  • 现有的蛋白质分析方法在吞吐量和细胞利用方面可能受到限制.

研究的目的:

  • 利用数字微流体学开发一种高通量,高复合的蛋白质测序方法.
  • 为了使单细胞蛋白质分析具有高效率和准确性.

主要方法:

  • 开发了一种使用DNA标记抗体和细胞条形码的数字微流体蛋白质测序 (DMF-Protein-seq) 系统.
  • 在数字微流体芯片上实施了分拆和聚合策略,以提高吞吐量.
  • 利用低吸附的疏水表面和隔离的反应空间来实现高细胞利用率.

主要成果:

  • DMF-Protein-seq系统实现了高复合,吞吐量和细胞利用率 (>90%).
  • 每个实验运行同时检测到大约1800个细胞,读取的映射率高 (>90%).
  • 成功地将蛋白质信息转化为用于单细胞分析的DNA测序读取结果.

结论:

  • 拟议的DMF-Protein-seq方法为单细胞蛋白质分析提供了一个敏感而高效的平台.
  • 这项技术具有基于蛋白质表达的细胞分类和药物监测的巨大潜力.
  • 数字微流体和DNA测序的整合克服了以前的吞吐量限制.