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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Jun 28, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

高空间分辨率的表面等离子体共振成像使用等离子体芯片.

Yasunori Nawa¹, Keiko Tawa¹

¹Department of Applied Chemistry for Environment, School of Biological and Environmental Sciences, Kwansei Gakuin University, 1 Gakuen Uegahara, Sanda, Hyogo 669-1330, Japan.

The Journal of chemical physics

|April 23, 2024

概括

这项研究引入了一种新的扫描格联表面等离子体共振 (GC-SPR) 成像仪器,用于高度敏感的生物分子检测. 这种先进的技术显著提高了在小区域的检测精度和灵敏度.

科学领域:

光学和光子学在光学和光子学.
生物感知技术的技术
纳米技术纳米技术

背景情况:

表面等离子体共振 (SPR) 对于无标签的生物分子量化至关重要.
传统的SPR方法由于背景噪声而面临检测区域和灵敏度的局限性.
在传统的SPR中,有限的检测区域和背景信号阻碍了高度敏感的检测.

研究的目的:

开发一种高空间分辨率的SPR方法,用于增强生物传感.
构建一个扫描格合SPR (GC-SPR) 成像仪器.
克服常规SPR对于敏感检测的局限性.

主要方法:

使用了扫描GC-SPR成像仪器,配备了高数值孔径目标镜头和等离子芯片.
采用聚焦到射极限的光来抑制背景信号.
通过扫描样本区域的焦点进行SPR成像.

主要成果:

在小区域 (<1μm2) 实现了高灵敏度检测.
高精度测量了水和二甲基硫氧化物混合物的折射率 (2.43 × 10-3 RIU).
成功评估了高灵敏度的超薄聚多巴胺薄膜 (<5 nm) 的厚度.

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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Published on: April 4, 2017

相关实验视频

Last Updated: Jun 28, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Published on: April 4, 2017

结论:

开发的扫描GC-SPR成像技术显著提高了检测灵敏度和准确性.
这种方法为分析微小的样本体积和超薄膜提供了强大的工具.
这一进步有望在各种生物传感应用中实现无标签,高度敏感的量化.