Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Facile biotic/abiotic sandwich detection system for the highly sensitive detection of human serum albumin and glycated albumin.

Analytical and bioanalytical chemistry·2024
Same author

High spatial resolution surface plasmon resonance imaging using a plasmonic chip.

The Journal of chemical physics·2024
Same author

A rapid abiotic/biotic hybrid sandwich detection for trace pork adulteration in halal meat extract.

Nanoscale·2023
Same author

Plasmonic coloration of silver nanodome arrays for a smartphone-based plasmonic biosensor.

Nanoscale advances·2022
Same author

Introduction to New era in advanced functional materials emerging from molecular imprinting and related techniques.

Journal of materials chemistry. B·2022
Same author

<i>In vivo</i> stealthified molecularly imprinted polymer nanogels incorporated with gold nanoparticles for radiation therapy.

Journal of materials chemistry. B·2022

相关实验视频

Updated: Jun 7, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.4K

使用等离子芯片和增强光显微镜进行单细胞外囊检测.

Kazuma Fukutomi1, Eri Fujimoto1, Masaya Shimokawatoko1

  • 1Graduate School of Science and Technology, Kwansei Gakuin University, 1 GakuenUegahara, Sanda, Hyogo 669-1330, Japan.

ACS omega
|November 11, 2024
PubMed
概括

这项研究引入了一种新的等离子体增强光方法,用于可视化和量化微小的细胞外囊泡 (EVs),用于疾病预测. 该技术超越了光学限制,使单个电动汽车能够在没有事先隔离的情况下进行敏感检测.

更多相关视频

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

7.6K
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

8.5K

相关实验视频

Last Updated: Jun 7, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.4K
Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

7.6K
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

8.5K

科学领域:

  • 生物技术是生物技术.
  • 纳米技术纳米技术
  • 医学诊断 医学诊断 医学诊断

背景情况:

  • 细胞外囊泡 (EVs) 是疾病预测的关键生物标志物.
  • 传统的光显微镜由于光学衍射极限,难以可视化100nm以下的EV.
  • 准确检测和量化单个电动汽车对于可靠的诊断至关重要.

研究的目的:

  • 开发一种可视化和量化单个细胞外囊泡 (EVs) 在光学衍射极限以下的方法.
  • 为了实现对EV的敏感检测,以改善疾病预测.
  • 为了区分单一的EV与非特定的绑定和聚合物.

主要方法:

  • 使用等离子芯片捕获单个电动汽车.
  • 使用等离子体场增强光与光标记抗体进行检测.
  • 分析了亮点特征 (FWHM,峰值值) 并使用传输光显微镜来区分EV.

主要成果:

  • 成功地检测和量化了皮科莫拉到女性摩尔范围 (1.4 pM95 fM) 的单个EV.
  • 通过同时标记两个不同的膜蛋白来证明EVs的检测.
  • 展示了在多个波长检测多个目标的能力,证实了特异性和灵敏性.

结论:

  • 增强等离子体光为单个EV检测提供了一种强大的,无标签的 (对于EV本身) 方法.
  • 该方法克服了光学限制,使纳米电动汽车可用于生物标志物发现的可视化.
  • 这种技术有望在基于细胞外囊分析的基础上推进敏感和特定的诊断工具.