对与胚胎肌肉发育相关的长非编码RNA的转录组分析.
1Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
British poultry science
|May 13, 2024
概括
这项研究研究了肉肌肉发育中的长非编码RNA (lncRNAs). 它确定了关键的lncRNA-mRNA相互作用,强调MSTRG.12395.2-FGFBP2和MSTRG.18590.6-FMOD对于胚胎肌肉生长至关重要.
科学领域:
- 动物遗传学动物遗传学
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 的骨肌肉发育对尸体质量至关重要,并且在胚胎时期在很大程度上确定.
- 北京 (BY) 和康沃尔郡 (CN) 的生长速度和体型不同,这使得它们成为研究肌肉发育的有价值模型.
研究的目的:
- 探索长非编码RNAs (lncRNAs) 在不同胚胎阶段的肌肉发育中的作用.
- 在胚胎发育过程中,在BY和CN品种之间识别差异表达的lncRNA和信使RNA (mRNA).
主要方法:
- RNA测序 (RNA-seq) 在11日,13日,15日,17日和1天大胚胎时从BY和CN的胸肌组织上进行.
- 对lncRNAs和mRNAs进行了系统分析,以确定差异表达的转录 (DET).
- 相关性分析被用来预测lncRNA-mRNA相互作用,并通过实验验证了关键对.
主要成果:
- 在五个发育阶段共鉴定了4,104个DET,包括2,359个lncRNA和1,745个mRNA.
- 在BY和CN品种之间基因调节的最大差异发生在胚胎第17天,1,658个lncRNA和1,016个mRNA被确定为差异表达.
- 预测了与肌肉发育相关的43种lncRNA-mRNA的cis相互作用对,并验证了MSTRG.12395.2-FGFBP2和MSTRG.18590.6-FMOD在ED11.11时在CN中显著上调.
结论:
- lncRNAs在调节的胚胎肌肉发育方面发挥着重要作用.
- 特定的lncRNA-mRNA相互作用,如MSTRG.12395.2-FGFBP2-FGFBP2和MSTRG.18590.6-FMOD,是肌肉生长潜力的潜在关键调节者.
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