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相关概念视频

CRISPR01:59

CRISPR

50.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
50.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
Experimental RNAi02:15

Experimental RNAi

6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K

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相关实验视频

Updated: Jun 26, 2025

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
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Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

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使用CRISPR-Cas13进行可编程RNA向.

Peiguo Shi1, Xuebing Wu1

  • 1Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA.

RNA biology
|May 20, 2024
PubMed
概括
此摘要是机器生成的。

该CRISPR-Cas13系统精确地准RNA,推进RNA调节研究和基于RNA的诊断和治疗. 本综述总结了Cas13工具,应用程序以及未来的发展方向.

关键词:
克里斯普尔-Cas13是什么意思这是一个RNARNARNARNARNA.担保活动担保活动.

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Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
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Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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相关实验视频

Last Updated: Jun 26, 2025

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
06:37

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Published on: February 5, 2021

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Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

272
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学是一种遗传学.
  • 生物技术是生物技术.

背景情况:

  • 该CRISPR-Cas13系统提供了内源RNAs的精确工程.
  • 这项技术显著提升了RNA调节的理解.
  • 它对于开发基于RNA的诊断和治疗至关重要.

研究的目的:

  • 要总结基于Cas13的RNA向工具和应用程序.
  • 讨论当前Cas13工具的局限性和挑战.
  • 建议RNA向系统的未来发展方向.

主要方法:

  • 关于CRISPR-Cas13系统的文献综述.
  • 分析当前的RNA向工具及其应用.
  • 确定挑战和未来的研究途径.

主要成果:

  • 多种Cas13RNA向工具的全面概述.
  • 详细检查研究和医学中的应用.
  • 确定关键的局限性,包括非目标效应和交付挑战.

结论:

  • CRISPR-Cas13是一种强大的RNA操纵平台.
  • 需要进一步发展,以克服现有的挑战.
  • 未来的研究应该专注于提高特异性,交付和扩大应用.