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相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

20.6K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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In-situ Hybridization02:31

In-situ Hybridization

9.4K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.4K

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相关实验视频

Updated: Jun 25, 2025

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
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Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

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通过combFISH进行多重复合在位RNA成像.

Yanxiu Liu1,2, Jiayu Chen1,2, Chen Lin3,4

  • 1School of Medicine, Huaqiao University, Xiamen, 361021, Fujian, China.

Analytical and bioanalytical chemistry
|May 22, 2024
PubMed
概括

我们开发了一种循环组合光在位杂交 (combFISH) 试验,用于多重RNA检测. 这种方法可以在组织中实现高通量空间基因表达分析,而无需通过结合化学测序.

关键词:
在位杂交RNA在位杂交RNA滚动圈放大器 滚动圈放大器一个单细胞分析.空间转录组学 空间转录组学

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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
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Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

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相关实验视频

Last Updated: Jun 25, 2025

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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • 多复合在位RNA成像对于高通量空间基因表达造型至关重要.
  • 现有的方法往往涉及复杂的协议,限制了它们的广泛采用.

研究的目的:

  • 提出一种新的循环组合性光 in situ 杂交 (combFISH) 试验.
  • 通过简化协议,使细胞培养和组织中的多重RNA检测成为可能.

主要方法:

  • 开发了一种循环组合光在位杂交 (combFISH) 试验.
  • 使用了从挂锁探头测试中生成的滚动圆形放大器.
  • 采用组合检测探头用于循环查询条码序列.

主要成果:

  • 在细胞培养和组织段中成功证明了多重RNA检测.
  • 该combFISH测定工作与新鲜冷和甲固定的嵌组织.
  • 该方法理论上允许在三个杂交周期中检测64个基因.

结论:

  • combFISH 试验提供了一种简单有效的方法,用于多重化 in situ RNA 成像.
  • 消除了按结合测序的化学性质,简化了协议.
  • 该方法有可能在研究和临床环境中得到广泛采用.