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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

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Updated: Jan 9, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

有效的空间基因表达造型利用分探针结合和滚动圆放大.

Xueqian Xia¹, Zhaoxiang Xie¹, Yu Yang¹

¹School of Medicine, Huaqiao University, Xiamen, Fujian, 361021, China.

|December 3, 2025

概括

此摘要是机器生成的。

一种新的局部RNA成像方法使用分分探针绑定和滚动圆放大 (RCA) 进行空间基因表达概况. 这种技术成功地绘制了小鼠脏中的基因模式,揭示了糖尿病的性别特异性变化.

关键词:

通过RNA成像进行RNA成像.在位杂交RNA在位杂交RNA 在现场测序测序.滚动圈放大放大滚动圈放大放大空间转录学空间转录学

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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

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Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Published on: January 19, 2017

相关实验视频

Last Updated: Jan 9, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Published on: January 19, 2017

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
生物技术是生物技术.

背景情况:

空间转录学使得RNA在原生细胞环境中的高分辨率映射成为可能.
目前的方法在现场捕获或图像RNA,保持基因调节研究的空间背景.

研究的目的:

引入一种用于空间基因表达造型的新型in situRNA成像方法.
在各种组织类型中展示强大的RNA检测和基因定型.
在健康和糖尿病小鼠脏中绘制空间基因表达模式.

主要方法:

开发了一种在现场RNA成像技术,利用分裂探头结合和滚动圆放大 (RCA).
分裂探头混合以向RNA,结合,形成圆形DNA模板,并通过RCA放大以增强信号.
结合成像技术和现场测序来分析82个基因.

主要成果:

该方法在组织切片和整体样本中实现了 robust in situ RNA 检测和基因定型.
82个基因的空间表达模式被绘制在小鼠脏中.
在近端管道和收集管道中观察到Aqp4的明显局部.
糖尿病脏的性别特异性转录组变化被发现具有空间分辨率.

结论:

分裂探针结合和基于RCA的方法为空间基因表达分析提供了强大的工具.
这种技术提供了对组织结构和细胞功能的高分辨率洞察.
这些发现突出了糖尿病中的性别特异性脏变化,为有针对性的研究铺平了道路.