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相关概念视频

Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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RNA Structure01:23

RNA Structure

71.4K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
71.4K
Nucleic Acids02:43

Nucleic Acids

44.1K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
44.1K
RNA Stability01:53

RNA Stability

33.5K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.5K
Nucleic acids02:43

Nucleic acids

161.8K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
161.8K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.6K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.6K

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相关实验视频

Updated: Jun 25, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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改性核酸和RNA结构预测

Yuliia Varenyk1, Ronny Lorenz2

  • 1Department of Theoretical Chemistry, University of Vienna, Vienna, Austria.

Methods in molecular biology (Clifton, N.J.)
|May 23, 2024
PubMed
概括
此摘要是机器生成的。

本研究展示了如何使用维也纳RNA包预测具有修饰基的RNA二次结构. 它结合了伪乌里丁和二乌里丁等修改,用于准确的结构和稳定性分析.

关键词:
能量定向的折叠方式最低的自由能源结构.修改后的核酸是什么低于最佳的结构.这是一种二氨酸二氨酸.这是一种伪乌里丁类药物.这是一个tRNARNA.

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RNA Secondary Structure Prediction Using High-throughput SHAPE
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

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相关实验视频

Last Updated: Jun 25, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

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RNA Secondary Structure Prediction Using High-throughput SHAPE
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 核酸修饰对于RNA结构,稳定性和功能至关重要.
  • 修改后的基因可以改变RNA结构空间和基因配对偏好.
  • 当前的RNA结构预测方法往往忽视了核酸修饰的影响.

研究的目的:

  • 展示一种将核酸修饰纳入RNA二次结构预测的方法.
  • 使用维也纳RNA包来计算修饰RNA分子的结构.
  • 分析特定修改,如伪乌里丁和二乌里丁,对RNA结构的影响.

主要方法:

  • 使用一个严重修改的转移RNA (tRNA) 分子作为模型系统.
  • 用维也纳RNA包来进行二次结构预测.
  • 计算最小的自由能量和低于最佳的结构与修改的基础支持.

主要成果:

  • 成功地将基因修改的影响整合到二次结构预测中.
  • 证明了考虑到特定修饰的RNA结构的计算,例如伪乌里丁和二乌里丁.
  • 展示了预测结构在不同程度的修改基含量的能力.

结论:

  • 提出的方法使得精确的RNA二次结构预测与修饰核酸.
  • 这种方法增强了对修改如何影响RNA结构和功能的理解.
  • 维也纳RNA包可以有效地用于模拟各种核酸修饰的影响.