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Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
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通过使用人类诱导的多能干干细胞衍生的心脏细胞来设计心脏微组织生物制造.

Kirk Butler1, Saif Ahmed1, Justin Jablonski2

  • 1Biomedical Engineering Department, Binghamton University, the State University of New York, Binghamton NY 13902.

bioRxiv : the preprint server for biology
|May 27, 2024
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概括

干细胞衍生后心脏细胞对于3D心脏组织模型至关重要. 低通道细胞产生更坚固的组织,促进心肌细胞功能,为心脏组织工程提供了潜力.

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这是心肌细胞 (cardiomyocytes).在心脏上,表心是表心.不同类型的相互作用.人类多能干细胞干细胞组织工程是组织工程.

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科学领域:

  • 心血管生物学 心血管生物学
  • 干细胞生物学 干细胞生物学
  • 组织工程是组织工程.

背景情况:

  • 脊心细胞对于人类心脏发育和体外心脏组织模型至关重要.
  • 诱导的多能干细胞为产生人类心表细胞提供了可再生的来源.
  • 由于可访问性限制,研究心上表皮发育和功能是具有挑战性的.

研究的目的:

  • 从iPSCs生成和表征心上细胞,用于心脏组织工程.
  • 评估细胞通道数对心上表皮细胞功能和3D微组织形成的影响.
  • 评估iPSC衍生表心细胞在与心肌细胞共同培养中的作用,以进行心脏组织重塑.

主要方法:

  • 微分子调节Wnt信号传输以诱导心表细胞从iPSCs分化.
  • 表心细胞种群的特征,包括WT1表达,形态和可扩展性.
  • 与心肌细胞一起形成3D微组织和共同培养系统,以评估功能整合和重塑.

主要成果:

  • 一个强大的心表细胞群 (大约. 87.7%的WT1+) 通过Wnt路径调制产生.
  • 低通道的上心细胞形成了更强大的3D微组织,并表现出不同的迁移子群.
  • 皮心细胞在共同培养中促进心脏组织重塑和迁移,而不会影响心肌细胞功能.

结论:

  • 来自干细胞的心脏表皮细胞可以可靠地生成和表征.
  • 早期经过的表心细胞是制造一致的工程心脏组织的最佳选择.
  • 这些发现提升了干细胞衍生表心细胞用于心脏建模和生物制造的潜力.