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Updated: May 5, 2026

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时长,千赫兹采样速率 活细胞中的三维单病毒跟踪 通过StayGold光蛋白融合实现

Yuxin Lin1, Jack Exell1, Haoting Lin1

  • 1Department of Chemistry, Duke University, Durham, North Carolina 27708, United States.

The journal of physical chemistry. B
|May 29, 2024
PubMed
概括

新的StayGold光蛋白可以在活细胞中进行长达一个小时的高速3D单个病毒跟踪 (SVT). 这一突破允许详细观察病毒动态和整个病毒生命周期.

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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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