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Updated: Jun 24, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

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人类Kir2.1通道:冷电磁数据处理和分子动力学模拟的协议

Carlos A H Fernandes1, Catherine Vénien-Bryan2

  • 1UMR 7590, CNRS, Muséum National d'Histoire Naturelle, IRD, Institut de Minéralogie, Physique des Matériaux et de Cosmochimie, IMPMC, Sorbonne Université, Paris, France. carlos.henrique_fernandes@sorbonne-universite.fr.

Methods in molecular biology (Clifton, N.J.)
|June 10, 2024
PubMed
概括

研究人员介绍了一种冷电子显微镜 (cryo-EM) 协议,用于确定Kir2.1通道的结构. 这种方法有助于理解离子通道功能,并开发相关人类疾病的治疗方法.

关键词:
低温电子显微镜的使用方法内部整合是积极的整合.基尔频道是基尔的频道.在 Kir2.1 里.分子动力学模拟的模拟.

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

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Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
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