Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.2K
5.2K
RNA Splicing01:32

RNA Splicing

56.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

3.1K
3.1K
Next-generation Sequencing03:00

Next-generation Sequencing

88.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.6K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

3.0K
3.0K
Alternative RNA Splicing02:18

Alternative RNA Splicing

3.8K
3.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The Rayleigh Quotient and Contrastive Principal Component Analysis II.

bioRxiv : the preprint server for biology·2026
Same author

Hybrid crosses reveal a cell-type-specific landscape of mouse regulatory variation.

bioRxiv : the preprint server for biology·2026
Same author

Uniform pre-processing of bacterial single-cell RNA-seq.

bioRxiv : the preprint server for biology·2026
Same author

Biophysical constraints on mRNA decay rates shape macroevolutionary divergence in steady-state abundances.

bioRxiv : the preprint server for biology·2025
Same author

<i>k</i> -spaces: Mixtures of Gaussian latent variable models.

bioRxiv : the preprint server for biology·2025
Same author

Determining gene specificity from multivariate single-cell RNA sequencing data.

bioRxiv : the preprint server for biology·2025
Same journal

conMItion: an R package adjusting confounding factors for associations in multi-omics.

Bioinformatics (Oxford, England)·2026
Same journal

SpaMFG: a Spatial Multi-omics Integration Method based on Feature Grouping.

Bioinformatics (Oxford, England)·2026
Same journal

CSCN: Inference of Cell-Specific Causal Networks Using Single-Cell RNA-Seq Data.

Bioinformatics (Oxford, England)·2026
Same journal

Sparse CCA-Based Mediation Analysis with High-Dimensional Exposures and Mediators.

Bioinformatics (Oxford, England)·2026
Same journal

Enhancing Cross-Context Generalization in Drug Perturbation Prediction with a Multimodal Conditional Diffusion Framework.

Bioinformatics (Oxford, England)·2026
Same journal

Primer Design through Submodular Function Estimation.

Bioinformatics (Oxford, England)·2026
查看所有相关文章

相关实验视频

Updated: Jun 23, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.7K

灵活的解析,解释和编辑技术序列与分割代码.

Delaney K Sullivan1,2, Lior Pachter2,3

  • 1UCLA-Caltech Medical Scientist Training Program, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, United States.

Bioinformatics (Oxford, England)
|June 14, 2024
PubMed
概括
此摘要是机器生成的。

splitcode 是一种用于处理序列阅读的新工具. 它在下一代测序数据中高效地解析,解释和编辑合成结构,如适配器和条码.

更多相关视频

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance
04:58

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

2.3K
Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
14:06

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

15.2K

相关实验视频

Last Updated: Jun 23, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.7K
Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance
04:58

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

2.3K
Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
14:06

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

15.2K

科学领域:

  • 基因组学和生物信息学
  • 分子生物学分子生物学

背景情况:

  • 下一代测序 (NGS) 库包含合成结构,如适配器,条形码和独特的分子标识符.
  • 这些序列对于精确解释测序试验结果至关重要.
  • 当它们包含实验信息时,处理和分析这些合成序列是必不可少的.

研究的目的:

  • 引入一款灵活高效的软件工具,用于解析,解释和编辑阅读序列.
  • 为各种测序试验的读数预处理提供多功能解决方案.

主要方法:

  • 开发分代码软件的开发.
  • 实现灵活的解析,解释和编辑功能,用于测序阅读.

主要成果:

  • 分解码允许对序列阅读进行高效和可重复的预处理.
  • 该工具支持来自广泛的单细胞和批量测序试验的库.

结论:

  • splitcode为研究人员提供了一个有价值的资源,用于下一代测序数据.
  • 该软件在序列阅读中促进了合成构造的简化分析.