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Updated: May 6, 2026

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基于Raman的PAT用于VLP降水:系统的数据多样化和预处理管道识别.

Annabelle Dietrich1, Robin Schiemer1, Jasper Kurmann1

  • 1Institute of Process Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.

Frontiers in bioengineering and biotechnology
|June 17, 2024
PubMed
概括

拉曼光谱能够实时监测病毒样粒子 (VLP) 沉,这是生物制药制造的关键步骤. 这个过程分析技术 (PAT) 工具量化了VLP和沉物,改善了过程理解和控制.

关键词:
拉曼光谱法 拉曼光谱法 拉曼光谱法化学测量方法 化学测量方法 化学测量方法部分最小平方回归.管道优化的管道优化预处理 预处理过程监控过程监控过程监控蛋白质沉是一种蛋白质沉.类似病毒的颗粒.

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Precipitation Titration: Endpoint Detection Methods01:19

Precipitation Titration: Endpoint Detection Methods

In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
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The experimental conditions in a gravimetric analysis should be optimized to maximize the particle size and purity of the obtained precipitate. Ideally, the concentration of the precipitating reagent should be low with effective stirring to maintain low relative supersaturation for the growth of large crystals. In homogeneous precipitation, the precipitant is slowly generated by a chemical reaction in the solution to avoid local reagent excesses. For example, urea decomposes gradually to...
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Types of Coprecipitation

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Sometimes, ions in a crystal lattice can undergo isomorphous replacement by inclusions of similar charge and size. For...
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Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...
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