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相关概念视频

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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相关实验视频

Updated: Jun 23, 2025

Generation of Genetically Modified Mice through the Microinjection of Oocytes
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用于基因操纵的技术.

Carla García López1,2, Luis Saralegui Remón1,2, Cristina Uruén García1,2

  • 1Unidad de Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.

Methods in molecular biology (Clifton, N.J.)
|June 17, 2024
PubMed
概括

本研究详细介绍了Streptococcus suis (S. suis) 遗传修饰的方法,重点关注DNA结构的制备和吸收技术. 这些协议对于在瑞士推进基因型和表型研究至关重要.

关键词:
基因工程是一种基因工程.变种突变者 变种突变者这是一种等离子体.这是一种Streptococcus suis.变革 变革 变革 变革

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Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 微生物学 微生物学

背景情况:

  • 瑞士杆菌 (S. suis) 是一个重要的病原体.
  • 分子和遗传技术对于研究S. suis的基因型和表型至关重要.
  • 对S. suis的基因组修改需要通过等位基因交换有效地获取DNA和染色体集成.

研究的目的:

  • 描述已建立的S. suis.基因组修改协议.
  • 为准备基因组构造和促进DNA吸收提供详细的方法.
  • 为了使研究人员能够对S. suis.进行遗传研究.

主要方法:

  • 使用克隆和重叠扩展PCR编制基因组构造.
  • 通过电穿孔和转化将DNA吸收到S. suis中.
  • 对参考S. suis菌株P1/7.7的协议的应用.

主要成果:

  • 成功实施所述的基因组构造准备协议.
  • 在S. suis.中证明了对DNA吸收的电解和转化功效.
  • 使用参考S. suis菌株P1/7.7进行协议的验证.

结论:

  • 描述的分子技术为S. suis的基因组修改提供了可靠的方法.
  • 这些协议是推动S. suis病原和遗传学研究的宝贵工具.
  • 成功的应用确保了研究人员能够有效地操纵S. suis基因组.