Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Homologous Recombination02:31

Homologous Recombination

50.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.4K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K
Base Excision Repair01:54

Base Excision Repair

22.3K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
22.3K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

3.5K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Rap1-mediated steric hindrance protects telomeres from MRX sensing.

Nature structural & molecular biology·2026
Same author

Rad51 determines pathway usage in post-replication repair.

Nature communications·2026
Same author

Copper Homeostasis is influenced by Ics3 in <i>Saccharomyces cerevisiae</i>.

microPublication biology·2025
Same author

Mechanism of trinucleotide repeat expansion by MutSβ-MutLγ and contraction by FAN1.

Nature communications·2025
Same author

Author Correction: Mechanism of BRCA1-BARD1 function in DNA end resection and DNA protection.

Nature·2025
Same author

The RecBC complex protects single-stranded DNA gaps during lesion bypass.

Proceedings of the National Academy of Sciences of the United States of America·2025

相关实验视频

Updated: Jun 23, 2025

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.2K

Rad51 确定在复制后修复中路径的使用情况.

Damon Meyer, Shannon J Ceballos, Steven Gore

    bioRxiv : the preprint server for biology
    |June 25, 2024
    PubMed
    概括
    此摘要是机器生成的。

    Rad51蛋白中的新突变揭示了它在DNA修复中的双重作用. 在Rad51中,使用了Rad51.

    更多相关视频

    Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
    06:44

    Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

    Published on: April 28, 2021

    4.0K
    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
    07:55

    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

    Published on: September 11, 2022

    1.8K

    相关实验视频

    Last Updated: Jun 23, 2025

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence
    07:55

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence

    Published on: June 26, 2020

    10.2K
    Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
    06:44

    Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

    Published on: April 28, 2021

    4.0K
    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
    07:55

    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

    Published on: September 11, 2022

    1.8K

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 生物化学 生物化学

    背景情况:

    • 停滞的复制分叉需要复制后修复 (PRR) 机制,包括同源重组 (HR),分叉回归和转化DNA合成.
    • Rad51蛋白对于基因组稳定性至关重要,它参与HR并保护停滞的叉子免受降解.
    • 规范PRR路径之间的选择仍然不完全理解.

    研究的目的:

    • 调查Rad51在复制后修复过程中调节途径使用中的作用.
    • 为了识别和描述Rad51中影响叉子保护但不影响重组的功能分离突变.
    • 阐明Rad51在停滞的复制分叉处理中的功能结构和机制基础.

    主要方法:

    • 在Saccharomyces cerevisiae Rad51.1.中分离功能突变的分离和表征.
    • 在体内和体外测试以评估重组能力,DNA结合,ATPase活性和叉子保护.
    • 高分辨率冷电子显微镜测定Rad51-ssDNA丝的结构.

    主要成果:

    • 确定了Rad51突变 (Rad51-E135D,Rad51-K305N) 具有正常的重组,但缺陷的分叉保护.
    • 突变者表现出改变的DNA结合特征,特别是对双链DNA (dsDNA) 的结合特征,影响ATPase活性.
    • 在实验室中,已证明Rad51对停滞叉的招募受损,并减少了dSDNA对核酶 (Dna2-Sgs1, Exo1) 的保护.
    • 确定了Rad51-ssDNA丝的高分辨率冷电子显微镜结构.

    结论:

    • 在停滞的复制分叉中,Rad51结合双重DNA的能力对于控制途径选择至关重要.
    • 在Rad51的分叉保护功能中的缺陷将复制后修复使用转向了替代的,潜在的致变性途径.
    • 这些发现为Rad51在维护基因组完整性方面的多方面的作用提供了机械的理解.