Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

4.8K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Liquid Chromatography coupled with Mass Spectrometry as an Analytical Strategy to Assess the Occurrence of Potentially Toxic Cyanogenic Glycosides in Edible Microgreens.

Plants (Basel, Switzerland)·2026
Same author

HILIC-ESI-HRMS amino acid profiling of fruits of barattiere (Cucumis melo L.), a traditional Apulian melon landrace.

Analytical and bioanalytical chemistry·2026
Same author

The multidimensional signature of oxysterols: Comparative RPLC-HRMS-based profiling of healthy and pancreatic tumour cell lines.

Talanta·2026
Same author

Overcoming challenges in LC separation of oxysterols through guided design space modelling: A comparison of three stationary phases.

Journal of chromatography. A·2026
Same author

Special Issue "Modern Analytical Strategies for Foodomics: From Nutritional Value to Food Security".

International journal of molecular sciences·2026
Same author

Bacterial-driven lipid remodeling in avocado fermentation via hydroxylation and oxidation routes.

Food chemistry·2025

相关实验视频

Updated: Jun 22, 2025

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

3.7K

微藻提取物用高分辨率质谱学的蛋白质学.

Mariachiara Bianco1, Cosima D Calvano2,3, Giovanni Ventura1,4

  • 1Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Bari, Italy.

Methods in molecular biology (Clifton, N.J.)
|June 28, 2024
PubMed
概括

两种蛋白质提取方法 Arthrospira platensis (spirulina) 使用质谱法 (MS) 进行了比较. 逆相液态染色学-电子喷射电离合合里埃变换质谱法 (RP-LC-ESI-FTMS/MS) 确定了100多种蛋白质,提供了全面的蛋白质组分析.

关键词:
蓝藻细菌是一种蓝藻细菌.DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA高分辨率质谱测量高分辨率质谱测量马尔迪 马尔迪 马尔迪 马尔迪蛋白质沉是蛋白质的沉.蛋白质组学是指蛋白质组学.螺旋藻是指螺旋藻中的一种.

更多相关视频

Analysis of Fatty Acid Content and Composition in Microalgae
07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

60.0K
A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii
07:51

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii

Published on: August 8, 2019

7.6K

相关实验视频

Last Updated: Jun 22, 2025

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

3.7K
Analysis of Fatty Acid Content and Composition in Microalgae
07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

60.0K
A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii
07:51

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii

Published on: August 8, 2019

7.6K

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 微藻生物技术 微藻生物技术
  • 分析化学 分析化学

背景情况:

  • 藻 (Arthrospira platensis) 是一种富含营养的微藻,含有大量的蛋白质.
  • 准确的蛋白质组分析对于理解螺旋藻的营养价值和优化其应用至关重要.
  • 现有的蛋白质提取方法需要对全面的蛋白质组学研究进行评估.

研究的目的:

  • 介绍和比较Arthrospira platensis的两个不同的蛋白质提取协议.
  • 评估不同质谱技术对螺旋藻蛋白质组概况的有效性.
  • 在螺旋藻样本中识别高质量的蛋白质,如 phycocyanins 和 phycoerythrins.

主要方法:

  • 使用水性Tris-HCl缓冲剂和冷协议提取蛋白质.
  • 从底部向上的蛋白质学方法涉及酶性消化.
  • 使用MALDI-TOF MS和RP-LC-ESI并联FTMS/MS与数据依赖采集 (DDA) 的质谱分析.

主要成果:

  • MALDI-TOF MS提供了快速的质指纹,但发现的蛋白质不到20个.
  • RP-LC-ESI并联FTMS/MS DDA识别了100多种蛋白质,从而实现了详细的定性蛋白质组分析.
  • 两种方法都在样本中证实了类似的高质量蛋白质配置文件,包括植物和植物,在样本中.

结论:

  • 与MALDI-TOF MS相比,RP-LC-ESI双重FTMS/MS DDA在全面的螺旋藻蛋白质组分析方面更优越.
  • 马尔迪-托夫MS作为一个有价值的工具,用于初步选的蓝藻细菌样本.
  • 这项研究证实了螺旋藻的始终高质量的蛋白质成分,不管环境的微小变化.