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相关概念视频

From DNA to Protein03:06

From DNA to Protein

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The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
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Structure of a Gene01:30

Structure of a Gene

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A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
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Synthetic Biology02:55

Synthetic Biology

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Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
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The Central Dogma01:20

The Central Dogma

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The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Gene Evolution - Fast or Slow?

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Updated: Jun 22, 2025

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
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从基因到产品的优化工作流程

Maya Yovcheva1, Kenneth W Thompson2

  • 1Thermo Fisher Scientific, Frederick, MD, USA. Maya.Yovcheva@thermofisher.com.

Methods in molecular biology (Clifton, N.J.)
|July 1, 2024
PubMed
概括
此摘要是机器生成的。

该ExpiSfTM表达系统允许高密度感染ExpiSf9TM细胞使用化学定义的,无动物来源的介质,以有效地产生重组蛋白和baculovirus.

关键词:
细菌病毒病毒的病毒.在化学上定义的定义.一致性 一致性 一致性基因转化为蛋白质的基因高密度的表达方式在Sf9细胞中,悬浮体的转化情况工作流的工作流.

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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 细胞培养技术 细胞培养技术

背景情况:

  • 重组蛋白表达对研究和治疗至关重要.
  • 传统的表达系统经常面临可扩展性和血清依赖媒体的挑战.
  • 对昆虫细胞培养有必要建立高效,明确的系统.

研究的目的:

  • 描述ExpiSfTM表达系统用于高密度昆虫细胞培养的工作流程.
  • 突出化学定义的,无动物来源的蛋白质表达介质的优点.
  • 在悬浮细胞中证明高效的转染和baculovirus生产.

主要方法:

  • 使用ExpiSfTM表达系统与ExpiSf9TM细胞.
  • 使用一种化学定义的,无血清,无蛋白质和无动物来源的介质.
  • 在培养物中进行高密度感染和直接生产baculovirus.

主要成果:

  • 该系统支持高密度感染ExpiSf9TM细胞.
  • 化学定义的介质促进了高效的传染和baculovirus生产.
  • 启动套件提供了1升培养装置的所有组件.

结论:

  • 该ExpiSfTM表达系统提供了一个多功能和高效的平台,用于重组蛋白质表达.
  • 它的无动物性质使其适用于生物制药应用.
  • 该系统简化了基于昆虫细胞的蛋白质和病毒生产的工作流程.