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Updated: Jun 22, 2025

Production and Purification of Baculovirus for Gene Therapy Application
06:11

Production and Purification of Baculovirus for Gene Therapy Application

Published on: April 9, 2018

15.0K

快速两天的细菌病毒定位使用流细胞计.

Maya Yovcheva1, Kenneth W Thompson2

  • 1Thermo Fisher Scientific, Frederick, MD, USA. Maya.Yovcheva@thermofisher.com.

Methods in molecular biology (Clifton, N.J.)
|July 1, 2024
PubMed
概括
此摘要是机器生成的。

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这项研究提出了一种快速的方法,使用光抗体染色和流细胞计量来量化baculovirus库存. 该技术通过在接种几小时后分析受感染的昆虫细胞来准确确定病毒标位.

科学领域:

  • 生物技术是生物技术.
  • 病毒学 病毒学
  • 细胞生物学 细胞生物学

背景情况:

  • 准确量化baculovirus库存对于在昆虫细胞培养和重组蛋白质生产中的可复制结果至关重要.
  • 传统的病毒定位方法可能是耗时和劳动密集的.

研究的目的:

  • 开发和验证基于快速流细胞计的方法来确定baculovirus标位.
  • 为了能够快速评估用于实验的baculovirus库存度.

主要方法:

  • 感染的 Spodoptera frugiperda (Sf9) 昆虫细胞与串行稀释的百科病毒库存.
  • 使用光标记的抗体对病毒gp64包膜蛋白进行染色感染细胞.
  • 使用流细胞计分析量化感染细胞.

主要成果:

  • 该方法允许量化感染baculovirus的昆虫细胞.
  • 标位是根据受感染细胞与未受感染细胞的比例计算的.
  • 结果可以在注射后13至18小时获得.

结论:

  • 这种快速检测方法提供了一种有效的方式来确定baculovirus标位.
关键词:
细菌病毒病毒的病毒病毒.流动细胞计量流动细胞计量定位 定位 定位 定位 定位

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  • 基于流细胞计的量化为传统定位方法提供了一种敏感和高通量替代方法.
  • 该试验适合在细菌病毒库存表征中常规使用.